Bacterial culture is commonly used in the preparation of cultures and antibiotics

I, commonly usedculturebase

1, LB

if necessary with 1NNaOH (-1ml) adjust pH to 7.0, and then fill the water to 1L. Note: < a href"">" agar plate needs to add agar powder 12g/L, upper agar plate to add agar powder 7g/L.

2, SOB culturer

water to replenish volume to 1L. Divide into small parts of 100ml and sterilise at high pressure. After the medium has cooled to room temperature, add 1 ml of sterilized 1mol/L magnesium chloride to every 100 ml of small servings.

3, SOC medium

4, TB culturer

components dissolve. Cool to 60 degrees C, add 100 ml of sterilized 170mmol/LKH2PO4/0.72mol/LK2HPO4 solution (2.31g KH2PO4 and 12.54gK2HPO4 dissolved in sufficient water, resulting in a final volume of 100ml. Autocultension or sterilization with a 0.22um membrane).

5, 2×YT culturer

if needed to adjust pH to 7.0 with 1NNaOH (-1ml), then fill the water to 1L. Note: Agar plate to add agar powder 12g/L, upper agar plate to add agar powder 7g/L.

6, YPD culturer

water to replenish the volume of 1L, autocultension. It is recommended to add 1.6 g tryptophan per liter of medium for tryptophan nutritional deficiencies prior to autocultension, as the YPD medium is a tryptophan-restricted medium. In order to make the plate, 20g agar powder needs to be added before autoculsion is sterilized.

2, commonly usedantibiotic

ampicillin (100mg/ml)

carbenicillin (50mg/ml)

methicillin (100mg/ml)
dissolves 1g of methicillin sodium in sufficient amounts of water, and finally settled to 10ml. It is packed into small parts and stored at -20 degrees C. It is often added to the growth culture with a final concentration of 37.5ug/ml with 100ug/ml ampicillin.

kanamycin (10mg/ml)
Dissolved 0.1g kanamycin in sufficient amounts of water, finally fixed to 10ml. It is packed into small parts and stored at -20 degrees C. It is often added to the growth culture at a final concentration of 10ug/ml to 50ug/ml.

chloramphenicol (25mg/ml)
Dissolved 0.25g chloramphenicol sufficient amount of waterless ethanol, finally fixed to 10ml. It is packed into small parts and stored at -20 degrees C. It is often added to the growth culture at a final concentration of 12.5ug/ml to 25ug/ml.

streptomycin (50mg/ml)
dissolves 0.5g streptomycin sulphate in sufficient amounts of waterless ethanol, finally fixed to 10ml. It is packed into small parts and stored at -20 degrees C. It is often added to the growth culture at a final concentration of 10ug/ml to 50ug/ml.

nalidixicacid (5mg/ml)
dissolved 0.05g sodium ketoneate in sufficient amounts of water, and finally settled to 10ml. It is packed into small parts and stored at -20 degrees C. It is often added to the growth culture at a final concentration of 15ug/ml.

tetracyline (10mg/ml)
dissolves 0.1g tetracycline hydrochlorate in sufficient amounts of water, or dissolves alkali-free tetracycline in waterless ethanol, to 10ml. Divided into small parts wrapped in aluminum foil filled with liquid tube to avoid the solution to see light, at -20 degrees C storage. It is often added to the growth culture at a final concentration of 10ug/ml to 50ug/ml.

Tips: If 10mg/ml canamycin is made, first in a salt water bottle orbottle to add 10 ml of double steamed water, high pressure, cooled to add 0.1g canamycin, mix well, filter membrane filter totest tube, and then 1 ml / tube into the EP tube for use.