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Detection of Mycoplasmas in Cell Cultures by Cultural Methods
Time of Update: 2021-02-12
However, mycoplasma contaminations of cell cultures are not as readily observed and are, therefore, the subject of a separate set of diagnostic tests.
However,Mycoplasma hyorhinis DBS 1050, a strain that commonly infects cell cultures, is not readily grown on the commonly used agar preparations ( 1 ).
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McKinney:TransformByElectroporation
Time of Update: 2021-02-12
Materials 50mL 7H9 mycobacterial medium + 3mL per transformation 102mL 10% glycerol (possibly a few mL more if you are doing many transformations) 400mL PBS + 0.1% Tween 80 per transformation electroporation cuvettes and electroporator LB agar plates carrying the relevant antibiotic markers frozen aliquot ofM.
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Recombinant Yeast mtDNA Helicases Purification and Functional Assays
Time of Update: 2021-02-12
Pif1 protein is a 5′->3′ DNA helicase that affects the stability of mtDNA at higher temperatures but is not essential at 28�C.
Pif1 is probably involved in mtDNA repair and recombination ( 1 , 2 ), but it also affects telomeres ( 3 ) and nuclear ribosomal DNA ( 4 ).
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Purification Strategies for Drosophila mtDNA Replication Proteins in Native and Recombinant Form: DNA Polymerase
Time of Update: 2021-02-12
Thus, the take-home lesson in the biochemical identification of new mitochondrial activity is to develop a differential assay and to optimize the yield of the mitochondria from which extraction is pursued.
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Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology
Time of Update: 2021-02-12
BACs are used in genetics of large DNA viruses such as herpes and baculoviruses for propagation and manipulation of complex genomic regions or even entire viral genomes in one piece.
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Automated Headspace Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry for Screening and Determination of Multiclass Pesticides in Water
Time of Update: 2021-02-12
The method for the determination of multiclass pesticides in water samples by headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC-MS) is described.
The automated HS-SPME-GC-MS system is an extremely powerful, flexible, and low-cost approach for multiclass pesticide analyses in various water samples.
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Biochemical and Enzyme Kinetic Applications for the Characterization of βLactamases
Time of Update: 2021-02-12
IEF is able to distinguish enzymes that focus only 0.05 p I apart ( 1 ), but the exponential increase rin the numbers of β-lactamases discovered over the last 10 yr has meant that this method no longer provides sufficient resolution to distinguish the majority of β-lactamases.
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In Vivo Study of Immunity to Rotaviruses: Selected Methods in Mice
Time of Update: 2021-02-12
The combination of two or three strategies (for example, passive cell transfer into immunodeficient hosts and immunodepletion of selected cell populations in immunocompetent hosts) have proven particularly useful in the study of immunity to RV.
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Speartail fish
Time of Update: 2021-02-12
Dorsal fin 2: the first dorsal fin is bone-strength, with a crucible, ratchet-like, and the second dorsal fin is similar in shape to the pectoral fin, abdominal fin and hip fin, and the fins are born on a very thick meaty fin handle.
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Use of Ribozymes to Specifically Alter the MDR-1 Phenotype
Time of Update: 2021-02-12
P-gp was found to be expressed in many types of multidrug resistant cells and was shown to be concentrated at the plasma membrane ( 7 – 9 ) The predicted transmembrane structure of the P-gp showed that it possesses 12 transmembrane domains that form a pore-like structure and has also two ATP-binding sites ( 10 , 11 ).
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Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts
Time of Update: 2021-02-12
It is only more recently, with the commercial availability of real-timePCR ( RT-PCR ) gene arrays that the expression profiles of up to 84 genes can be carried out simultaneously.
The RNA is then reverse transcribed to c DNA and analyzed using the RT-PCR focused-gene arrays.
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The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies
Time of Update: 2021-02-12
fulcipurum has been studied in culture ( 7 ) and biochemical and immunochemical studies have been done to identify and characterize sexual stage specific proteins, especially those that are targets of transmission blocking antibodies ( 5 ).
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Frozen river 鲀 fish
Time of Update: 2021-02-12
process Raw materials → raw materials treatment→ cleaning→ weighing plate →freezing→ refrigeration production method 1.
Raw material selection: raw materials for processing river fish necessary fresh.
2. Raw material treatment: when processing, cut the head, remove all internal organs.
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Isolation of Bacteria Envelope Proteins
Time of Update: 2021-02-12
Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques.
After extensive testing of various protocols for the preparation of envelope contents, we found that a modified version of the method of Oliver and Beckwith consistently produces the cleanest extract of periplasmic and outer membrane proteins.
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Myophysics basic knowledge and detection methods: myosome culture technology!
Time of Update: 2021-02-12
basic knowledge and detection methods: myophysics, training technology! mycomal also known as mold, is a very small primary nuclear cell, completely without cell walls. The shape of the bacterium
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Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: Deletion of gal7
Time of Update: 2021-02-12
As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications that require creation of designer strains of this yeast.
In particular, it has various potential applications in metabolic engineering, systems biology, synthetic biology, and molecular genetics.
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Molecular-Based Identification and Typing of Campylobacter jejuni and C. coli
Time of Update: 2021-02-12
Thermophilic Campylobacter spp., mainlyCampylobacter jejuni and to a lesser extentC. coli are recognized as the most common bacteriological causes of gastroenteritis in humans ( 1 ). As enteric in
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Studier Lysate Prep
Time of Update: 2021-02-12
I often use LB --Sri Kosuri 15:18, 3 Jun 2005 (EDT) Add a single plaque to above (or a drop of lysate) Shake at 30°C until lysis is visually apparent (2-3hr) As soon as lysis is observed and appears to be complete, add 1g of NaCL to the flask and dissolve by shaking.
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Lipopolysaccharide Chemotyping
Time of Update: 2021-02-12
Lipopolysaccharide (LPS), traditionally termed somatic antigen or O-antigen, forms an integral part of the outer membrane of Gram-negative bacteria.
A hydrophobic portion, lipid A, which is composed of long-chain fatty acids and which anchors LPS molecules into the outer membrane; 2.
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Mutagenesis of Herpesvirus BACs by Allele Replacement
Time of Update: 2021-02-12
Therefore, we have introduced a negative selection marker into the shuttle plasmid (thesacB gene) that allows to select against the bacteria still containing a nonresolved cointegrate (see Fig. 1 , step 4) and to identify bacterial clones harboring the mutant or the parental BAC.
