The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies
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Last Update: 2021-02-12
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Source: Internet
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Author: User
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Since the development of methods for in vitro cultivation of the asexual blood stages of
P.faZciparum
(
1
) and the production of mature gametocytes from such cultures capable of infecting mosquitoes (
2
), it has been possible to conduct a wide range of studies on the sexual stages of
P. filciparum
. These include studies on the infectivity of the gametocytes of this parasite to mosquitoes (
3
) and the biology of the parasites in the vector (
4
) including studies on transmission blocking immunity using monoclonal antibodies against gamete surface antigens (
5
) and human sera following natural malarial infections (
6
). The developmental origin of sexual stages of
P. fulcipurum
has been studied in culture (
7
) and biochemical and immunochemical studies have been done to identify and characterize sexual stage specific proteins, especially those that are targets of transmission blocking antibodies (
5
). The genes encoding several sexual stage specific proteins have been identified and fully sequenced (
8
–
10
).
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