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Retroviral Vectors as Insertional Mutagens
Time of Update: 2021-02-12
In addition to these direct as-acting effects, there can be indirecttrans -regulatory effects resulting from the presence of the viral genome within the cell, but irrespective of its position of integration.
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Preparation of DNA and RNA from Trypanosoma brucei
Time of Update: 2021-02-12
The use of VSGcDNA s to probe Southern blots of genomic DNA provided the demonstration that in most cases antigenic variation is the result of the duplicative transposition of a VSG gene to an expression site ( 6 – 8 ).
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Cloning of β-Herpesvirus Genomes as Bacterial Artificial Chromosomes
Time of Update: 2021-02-12
This is especially true for the members of the β-herpesviruses, i.e., the cytomegaloviruses (CMVs), because their slow replication kinetics, cell association, and large genome size (230 kb) made the construction of viral mutants a difficult and tedious procedure ( 2 ).
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Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
Time of Update: 2021-02-12
The present protocol can also be used for “multiplexing,” i.e. the analysis of several samples in a single flowcell lane by generating “barcoded” or “indexed” Illumina sequencing libraries in a way that is independent from Illumina-supported methods.
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The Relationship between Helicobacter Pylori Infection and Remnant Gastritis in 152 Cases
Time of Update: 2021-02-12
After partial gastric excision, 60% to 100% of patients developed residual gastritis, and the rate of HP-positive detection in the residual stomach was reported at 25% to 71%.
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Investigation of Staphylococcus aureus Adhesion and Invasion of Host Cells
Time of Update: 2021-02-11
aureus can invade different types of host cells, which may contribute to escape from host immune defense as well as evade the toxicity of certain antibiotics.
In this study, we found that different human MRSA isolates possessed different abilities to adhere to and invade the epithelial cells.
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Firefish
Time of Update: 2021-02-11
firefish that live in the waters of Oceania's Solomon Islands and are known for their "explosion of Mars." Whenever they travel in groups, their bodies collide and rub against each other, and sparks pop up "and shoals" that look like the electric light emitted when the positive and negative electrodes hit each other.
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The New Year is approaching - remember the outbreak protection, probiotics to help!
Time of Update: 2021-02-10
For example, The star strain of Coto Bio, Cheese Cheese Bacillus Zhang, Milk Bifidobacteria V9 and Plant Lactobacillus P-8, etc., can be very good to regulate the intestinal flora, maintain the balance of the flora, increase the content of beneficial bacteria, reduce the content of pathogenic bacteria, improve the body's immunity, reduce related disease infections, effectively prevent bacterial infection.
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Probiotics regulate gut microorganisms to relieve mental anxiety during crew sailing
Time of Update: 2021-02-10
The results showed that after the voyage, 42 seafarers who took placebo had intestinal bacteri group disorders, increased levels of pathogenic bacteria (Gordon's Streptococcus and Pneumonia Kreber), and the crew's psychological stress and mental anxiety were at risk.
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Probiotics regulate the gut microbiome to relieve stress and anxiety symptoms in humans
Time of Update: 2021-02-10
Overall, P-8 promotes the production and secretion of some key neurotransmitters and neuro-actives in the intestine by regulating the gut bacteriome, and directly and indirectly regulates the intestinal-brain-axis through the ecstransic nerves, cytokines, and microbial-sourced metabolites, thus relieving adult stress and anxiety .
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Professor Zhang Peace of Inner Mongolia expounds the research progress of probiotic fermented milk and its in vivo function.
Time of Update: 2021-02-02
Therefore, the literature in this review focuses on in vivo studies and clinical trials that have used probiotic fermented milk since 2015 to treat a variety of pathophysiological diseases and cognitive impairments.
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Can probiotics also relieve stress anxiety? Probiotic leader Koto Bios amplifys again
Time of Update: 2021-02-02
Koto biological research confirmed that plant Lactobacillus P-8 can enhance the nerve stimulation activity of the intestinal flora to relieve stress and anxiety, the relevant research results in January 2021 in the international authoritative journal "Neurobiology of Stress" successfully published, which represents the research has been recognized by the international authoritative field, is China's probiotic industry in the field of mental health research a major breakthrough, to advance to the international leading level.
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Development of Serum-Free Media for Lepidopteran Insect Cell Lines
Time of Update: 2021-02-01
Hence, there is wide appeal for formulating serum-free media based on a rational assessment of the metabolic requirements of the lepidopteran cells during both the growth and the production phases.
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Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA
Time of Update: 2021-02-01
The augmentation ofDNA delivery by adenovirus particles is primarily due to enhancement of cytoplasmic entry of DNA; however, viral components may also serve additional functions that promote transfection, such as generating alterations in the cytoskeleton of the transfected cell ( 8 ) or in promoting nuclear entry ( 9 , 10 ).
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Methods for Treating HIV by Gene Therapy Using Ribozymes
Time of Update: 2021-02-01
Ribozymes are small catalytic RNAs with the ability to reversibly cleave covalent bonds in RNA in the complete absence of protein ( 1 ).
The first ribozyme to be identified was the self-splicing ribosomal RNA precursor of the protozoanTetrahymena ( 2 , 3 ).
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Available Lepidopteran Insect Cell Lines
Time of Update: 2021-02-01
This chapter lists the known cell lines from Lepidoptera, largely based on previous compilations of insect cell lines published by W. Fred Hink. The official designation is given for each cell line a
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Chlamydia psittaci
Time of Update: 2021-02-01
Parrot chlamydia is easy to grow in chicken embryo yolk sacs and HeLa cells, monkey renal cells culture and can infect mice with pneumonia, periaryitis or encephalitis and die.
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Isolation of genomic DNA from bacteria
Time of Update: 2021-02-01
Precipitate nucleic acids by adding 25 mL 5 M NaCl and 1 mL 95 EtOH, vortex, centrifuge for 10 min, decant supernatant.
Resuspend pellet in 100 mL TE buffer, add 5 mL RNaseA (5 mg/mL in TE), incubate at 37oC for 30 min.
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Immobilization of Enzymes by Covalent Attachment
Time of Update: 2021-02-01
Enzymes are finding increasing use for the production of agrochemicals, pharmaceuticals, and fine chemicals.
In addition, immobilization often enhances the stability of the enzyme.
This chapter discusses various methods, properties, and uses of covalently immobilized enzymes.
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Minimization and Prevention of Phage Infections in Bioprocesses
Time of Update: 2021-02-01
Phage infections in bacterial bioprocesses constitute one of the most devastating threats to the productivity of the biotechnology facilities.
There are several factors, which can decide if an infection would occur, and if it would turn into an outbreak and heavy contamination of the production facility.
