 Home > Biochemistry News > Microbiology News > Library Preparation and Data Analysis Packages for Rapid Genome Sequencing

Library Preparation and Data Analysis Packages for Rapid Genome Sequencing

 Last Update: 2021-02-12
 Source: Internet
 Author: User

Tags

survey data analysis

single cell analysis

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

High-throughput sequencing (HTS) has quickly become a valuable tool for comparative genetics and genomics and is now regularly carried out in laboratories that are not connected to large sequencing centers. Here we describe an updated version of our protocol for constructing single- and paired-end Illumina sequencing libraries, beginning with purified genomic
DNA
. The present protocol can also be used for “multiplexing,” i.e. the analysis of several samples in a single flowcell lane by generating “barcoded” or “indexed” Illumina sequencing libraries in a way that is independent from Illumina-supported methods. To analyze sequencing results, we suggest several independent approaches but end users should be aware that this is a quickly evolving field and that currently many alignment (or “mapping”) and counting algorithms are being developed and tested.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Meat extracts, beef

CAS No.: 68990-09-0

Industrial Grade or Medical grade

$1/MT EXW

Fecal coliform positive control samples (multi-tube fermentation) BNCC359094 BNCC

CAS No.:

$220-280/MT EXW

Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method) BNCC360407 BNCC

CAS No.:

$220-280/MT EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.