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Methods for Treating HIV by Gene Therapy Using Ribozymes

 Last Update: 2021-02-01
 Source: Internet
 Author: User

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2 3 dpg

16s ribosomal rna

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Ribozymes are small catalytic RNAs with the ability to reversibly cleave covalent bonds in RNA in the complete absence of protein (
1
). With the exception of the RNA component of bacterial RNase P, presently characterized ribozymes catalyze a one-time intramolecular self-cleavage in their natural settings (
1
–
9
). However, they can be engineered to act
in trans
in a truly catalytic manner to cleave multiple copies of a specific RNA target molecule. The first ribozyme to be identified was the self-splicing ribosomal RNA precursor of the protozoan
Tetrahymena
(
2
,
3
). Several other classes of RNA-cleaving RNAs have since been discovered (
1
. Investigations of ribozymes have begun to yield diverse outcomes that range from novel hypotheses about pre-biotic evolution (
7
) to potential human gene therapies (
10
,
11
)

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