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Study on the Mechanism of Xilei Powder in Treating Peptic Ulcer
Time of Update: 2021-02-14
table 2 effects of tin dispersion on PGE2 content of gastrointestinal mucous membrane tissue in patients (ng/l, x±S) group n pre-treatment treatment control 20 615±12.9 DU 42 edge 49 1.4±32.1 648.8±36.2b Gastric Sinuses 512.7±11.7a655.8±42.2b
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Demonstration of Extrachromosomal Elements
Time of Update: 2021-02-14
Mycoplasmas are parasites and pathogens of plants, insects, and animals, including humans. Although mycoplasmas are highly evolved parasites, they are not immune to being parasitized themselves. With
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Measurement of Natural-Killer Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells
Time of Update: 2021-02-14
Natural-killer (NK) cells are lymphocytes that do not express the CD3 T-cell receptor but do express the CD16 (FcγRIII) and CD56 (isoform of NCAM) in humans or NK1.1 antigen in certain strains of mice.
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Public Health Implications Related to Spread of Pathogens in Manure From Livestock and Poultry Operations
Time of Update: 2021-02-14
During the 20th century, food animal agriculture grew from small operations, where livestock (cattle, sheep, and swine) and poultry (chickens and turkeys) had access to free range, to large operations where animals and poultry were concentrated and confined to feed lots or buildings.
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How to Deposit Biological Material for Patent Purposes
Time of Update: 2021-02-14
To assure reworkability of an invention where biological material is involved, a sample should be deposited with an independent, recognized patent depositary.
Also demonstrated is how third parties can obtain the biological material that has been deposited for patent purposes.
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Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle
Time of Update: 2021-02-14
The newer cryo-electron microscopy technique, which combined with advanced image processing tools has recently yielded an atomic resolution picture of the complete virion, is also described.
Finally, we detail the procedure for imaging and interacting with single adenovirus virions using the atomic force microscope in liquid conditions.
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Expression Vectors for the Construction of Hybrid Ty-VLPs
Time of Update: 2021-02-14
Purification can be facilitated by expressing the recombinant protein as a fusion with a carrier protein that assembles into particulate structures.
Additional protein coding sequences can be fused to the carrier protein gene and expressed in yeast to produce hybrid Ty-VLPs ( 3 , 4 ).
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Confocal Laser Scanning Microscopy of Environmental Samples
Time of Update: 2021-02-14
Enormous technical advances in imaging and data acquisition techniques, combined with a continuing increased scope for fluorescence labeling of specific constituents of living organisms, have brought about a revolution in approaches to biological problems.
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鲌
Time of Update: 2021-02-14
For carnivorable fish, food types vary according to individual size, and the average young fish eats branches, pods and aquatic insects; The breeding period is generally to 7 , spawning in still or flowing water.
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Development of a Capillary Waveguide Biosensor Analytical Module for Use with the MBARI Environmental Sample Processor
Time of Update: 2021-02-14
We have developed a capillary waveguide biosensor (CWB) which employs nucleic acid hybridization for detection and quantification of specific microorganisms, and have integrated it with the MFB for use with the ESP.
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Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions/Gene Replacements in the Major Late Transcription Unit
Time of Update: 2021-02-14
The capsid proteins hexon, penton, and fiber contribute to efficient infection by adenovirus, and each contributes in some manner to the antiviral immune response against adenovirus infection.
In this chapter, we are presenting a terminal exon-replacement strategy that can be used to genetically manipulate capsid proteins expressed from the MLTU.
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Purification of Recombinant Protein Derived fkom the Baculovirus Expression System Using Glutathione Affinity Agarose
Time of Update: 2021-02-14
Viruses from the Baculoviridae subfamily of insect viruses, usuallyAutographa californica nuclear polyhedrosis virus (AcNPV), are most frequently used because they contain very active late gene promoters for proteins that are expressed late in the virus life cycle and are not essential for virus replication.
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Mouse Models for Studying Orthopoxvirus Respiratory Infections
Time of Update: 2021-02-14
The popular small-animal orthopoxvirus models employ the inbred mouse as the host, the respiratory tract as the site of virus inoculation, and orthopoxviruses—vaccinia, cowpox, and ectromelia viruses—as surrogates for variola virus.
This chapter describes an ectromelia virus respiratory infection model in the mouse.
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Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins
Time of Update: 2021-02-14
Previous bioassays used to quantitate adenoviruses (such as the plaque assay or fluorescent focus assay) are time-consuming and subjective in their interpretation.
Here we describe a flow cytometric method that eliminates these disadvantages and provides a quantitative and reliable method of focus-forming unit (FFU) assay.
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Spoon kiss
Time of Update: 2021-02-14
spoonfuls of kissing catfish is a famous large freshwater economic fish produced in North America (United States), its food is similar to that of our country's mackerel, zoolibacy as bait, is a filter fish, but can also feed water and artificial co-feed.
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Herring
Time of Update: 2021-02-14
Usually inhabits the middle and lower layers of water, generally in the bottom of the larger body of water.
After reproduction is often concentrated in river bends, lakes and ancillary bodies of water fertilization, winter in the deep water of the riverbed winter.
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Methods for the Detection and Concentration of Bacteriocins Produced by Lactic Acid Bacteria
Time of Update: 2021-02-14
Lactic acid bacteria (LAB), used for centuries by man to preserve food, produce a wide variety of antagonistic compounds, including lactic acid, hydrogen peroxide, and bacteriocins.
Bacteriocins are antimicrobial peptides that are bactericidal toward bacteria taxonomically close to the producer ( 1 ).
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Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB
Time of Update: 2021-02-14
Gene replacement and transposon mutagenesis are two complementary tools that have been widely used to perform genetic studies in various living organisms. In mycobacteria, and especially in theMycoba
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Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps
Time of Update: 2021-02-14
We have developed a heterologous membrane protein expression system that can be used to study the structure and function of these proteins in the non-pathogenic, genetically stable, and versatile eukaryotic model organism,Saccharomyces cerevisiae .
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Mapping DNA Interaction Sites of Chromosomal Proteins: Crosslinking Studies in Yeast
Time of Update: 2021-02-14
Only sequences specifically bound by the regulatory protein in vivo coimmunoprecipitate with this factor (the shaded area in the P1/P2 region symbolizes an interaction site) and will give rise to PCR products from the PRECIPITATE(F) .
