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    Home > Biochemistry News > Microbiology News > Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps

    Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps

    • Last Update: 2021-02-14
    • Source: Internet
    • Author: User
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    Azole antifungal drugs are used widely to treat people with oral fungal infections. Unfortunately, fungi can develop resistance to these drugs. This resistance can be due to the overexpression or mutation of cytochrome P450 14α-lanosterol demethylase, also known as
    ERG11
    or
    CYP51
    , and/or the overexpression of membrane-located multidrug efflux pumps. We have developed a heterologous membrane protein expression system that can be used to study the structure and function of these proteins in the non-pathogenic, genetically stable, and versatile eukaryotic model organism,
    Saccharomyces cerevisiae
    . In this chapter we describe the techniques used to express the
    Candida albicans
    efflux pump Cdr1p in
    S. cerevisiae
    .
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