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Gene Replacement and Transposon Delivery Using the Negative Selection Marker sacB

 Last Update: 2021-02-14
 Source: Internet
 Author: User

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tuberculosis

gene delivery

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Gene replacement and transposon mutagenesis are two complementary tools that have been widely used to perform genetic studies in various living organisms. In mycobacteria, and especially in the
Mycobacterium tuberculosis
complex, the lack of these tools has severely hampered the genetic studies of these organisms, especially regarding the identification and characterization of virulence genes of these important pathogens.

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