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Examination of Bacillus anthracis Spores by Multiparameter Flow Cytometry
Time of Update: 2021-02-24
The ability to rapidly differentiateBacillus anthracis spores from spores belonging to otherBacillus spp.
anthracis strains are fully virulent and the ability to determine the potential virulence of the endospore is also important.
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Enterococci
Time of Update: 2021-02-24
Lancefield’s group D is composed of Streptococcus equinus, S.
faecalis, S.
bovis , andS.faecium.
S. avium (Q and D antigens).
Group D is found in the intestinal tract of warm-blooded animals.
The term “enterococcus” refers only to the speciesS.
faecalis andS.faecium .
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Characterization of Carotenogenesis Genes in the Cyanobacterium Anabaena sp. PCC 7120
Time of Update: 2021-02-24
PCC 7120), we have produced gene-disruption mutants lacking selected proposed carotenoid biosynthetic enzymes.
Double-reciprocal recombination replaces the target genes inAnabaena genome with mutated ones on the plasmid.
Carotenoids in the selected double recombinants are identified using high-performance liquid chromatography.
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Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR
Time of Update: 2021-02-24
Various strategies have been used to develop DNA probes for specific bacteria that include the use of genes for antigenic determinants and virulence factors, randomly cloned DNA fragments, high copy number sequences including insertion elements, and ribosomal RNA (rRNA) genes ( 1 – 7 ).
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Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans
Time of Update: 2021-02-24
C. albicans biofilms often lead to life-threatening systemic infections and are particularly difficult to eradicate because of their high levels of resistance to antibiotics.
albicans biofilm cells, perhaps rendering biofilms more sensitive to antibiotics.
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Field Study Methods for the Determination of Bystander Exposure to Pesticides
Time of Update: 2021-02-24
Techniques to estimate bystander exposure are described.
Passive sample media such as filter paper are used to collect spray drift.
Air-sampling devices are used to determine the airborne concentration of pesticides.
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PCR-DNA Sequence Typing
Time of Update: 2021-02-24
There is a great need to develop molecular techniques for the typing ofHelicobacter pylori isolates since classical bacteriological assays, such as serotyping or lysotyping, are lacking. Such techni
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Animal Models for Mitochondrial Disease
Time of Update: 2021-02-24
Although a variety of degenerative diseases are now known to be caused by two mutations in mitochondrial genes, the pathophysiology of these diseases remains poorly understood.
As a consequence, relatively little progress has been made in developing new therapies for mitochondrial diseases.
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Selection of Hybrids by Differential Staining and Micromanipulation
Time of Update: 2021-02-24
In addition, genetic markers introduced into parent strains for convenience in the selection of mutants may cause practical problems; a new gene once introduced may produce unnecessary metabolites and thereby disrupt the metabolism of the cell, use energy that could be used for growth, or consume a substance that would otherwise be a source of the desired product ( 1 ).
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Immunoprecipitation of E1 A-Containing Protein Complexes
Time of Update: 2021-02-24
Important insights into cell growth and transcriptional regulation have been realized by correlating adenovirus ElA-induced cell-growth-control mechanisms with ElA’s specific interactions with host-cell proteins ( see ref.
A key method for directly demonstrating these ElA-host cell protein interactions is by immunoprecipitation.
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Identification of Microorganisms Using Random Primed PCR
Time of Update: 2021-02-24
The first method depends on a knowledge ofDNA sequence unique to the organism under study and provides a specific means of identifying that organism.
The second method has an absolute requirement for a pure culture of the target organism but requires no knowledge whatsoever of the DNA sequence of that organism.
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Can intestinal bacteria regulate our blood cholesterol levels?
Time of Update: 2021-02-23
, the presence of gut bacteria is critical to the stability of cholesterol throughout the body, strongly regulating blood cholesterol levels, liver cholesterol synthesis, and intestinal liver circulation.
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Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
Time of Update: 2021-02-23
Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented.
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Spring Festival dietary precautions
Time of Update: 2021-02-23
Suitable timely and appropriate meals to avoid overeating During the Spring Festival, many people often play late into the night or even overnight, the diet law is completely disrupted, coupled with food rich, emotional excitement, it is easy to overeat, or even overeating, thereby harming health.
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A healthy diet after the festival relieves gastrointestinal discomfort
Time of Update: 2021-02-23
Coupled with the Spring Festival diet pay attention to rich, big fish meat is inevitable, so the stomach after a few days of overload operation is easy to produce problems.
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Transformation of Lactococcus by Electroporation
Time of Update: 2021-02-22
lactis employed protoplast transformation in the presence of polyethylene glycol ( 1 ).
Later work indicated that the cell wall is a physical barrier to the entering DNA not only during protoplast transformation, but also during electroporation.
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Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers
Time of Update: 2021-02-22
The standard approach for the isolation of vaccinia virus recombinants involves homologous recombination between a transfected plasmid and the replicating viral DNA .
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Neutralization of the In Vivo Activity of E. Coli-Derived Lipopolysaccharide by Cationic Peptides
Time of Update: 2021-02-22
Lipopolysaccharide-binding and neutralization of its biologic activity can be detected and quantified by monitoring specific in vivo responses induced by intravascular introduction of lipopolysaccharide into conscious, instrumented animals.
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Quality Criteria in Pesticide Analysis
Time of Update: 2021-02-22
There is an increasing concern in pesticide residue analysis laboratories to ensure the quality of their analytical results.
Internal quality control (I QC ) measures are an essential element to ensure reliable results because they allow both the continuous monitoring of the process and measurements and the elimination of causes of unsatisfactory performance.
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Design and Construction of Poliovirus Epitope Expression Vectors
Time of Update: 2021-02-22
We have developed the very safe and efficacious live-attenuated Sabin 1 poliovirus vaccine strain as a vehicle for the presentation of defined epitopes from foreign pathogens ( 1 – 3 ). Precise modif
