Immunoprecipitation of E1 A-Containing Protein Complexes
Last Update: 2021-02-24
Search more information of high quality chemicals, good prices and reliable suppliers, visit
Important insights into cell growth and transcriptional regulation have been realized by correlating adenovirus ElA-induced cell-growth-control mechanisms with ElA’s specific interactions with host-cell proteins (
). A key method for directly demonstrating these ElA-host cell protein interactions is by immunoprecipitation. This assay is performed under conditions that efficiently lyse cells, but preserve protein-protein interactions. This method combines the elegant specificities of monoclonal antibodies and the fortuitous properties of nonionic detergents. These highly efficient and specific means of isolating protein complexes from cell lysates, while detailed here for E1Acontaining protein complexes, have general application for the study of other cellular-protein complexes.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to firstname.lastname@example.org
. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.