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Assay Systems for Measurement of Chemotactic Activity
Time of Update: 2021-02-13
Of the antibacterial proteins present within the human PMN, cationic antimicrobial protein ofM r 37 kDa (CAP37) and the defensins have been shown to have chemotactic activity for monocytes ( 3 ,4 ).
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Yeast Fluorescence Microscopy
Time of Update: 2021-02-13
Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells.
These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrialDNA , and two different procedures for investigation of yeast cells by immunofluorescence.
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Critical Aspects of Standardization of PCR
Time of Update: 2021-02-13
Molecular methods, using nucleic acid diagnostics, are receiving increasing attention for testing the microbiological safety of food ( 2 ).
There are several advantages of nucleic acid diagnostics compared to bacteriological culturing and immunochemical methods.
Further-more, detection of nucleic acids is very rapid compared to conventional culture-based analysis.
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Quality-Control Testing of Mycoplasma Medium
Time of Update: 2021-02-13
Details of components added to any particular medium will vary, according to the species of mycoplasma it is intended to culture ( see Chapters 4 –6 ).
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Inducible Degron and Its Application to Creating Conditional Mutants
Time of Update: 2021-02-13
In this chapter, a method is described that allows for a rapid design-based generation of temperature-sensitive alleles of manySaccharomyces cerevisiae genes.
The method employs a temperature-inducible degron, denoted astd , which, when linked to the N-terminus of proteins to be studied, targets them for rapid degradation via the ubiquitin-dependent N-end rule pathway.
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Frozen shrimp
Time of Update: 2021-02-13
process Raw materials → (unfrozen→ peeled meat →washed→ graded→ steamed→ cooled→ packed →frozen→ →stacked→ Production method 1.
When the cooked shrimp is spread well on the cooling table and the temperature drops to a cold feel, it is fed into the packaging room.
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Identification and Analysis of Native Nucleosomal Histone Acetyltransferase Complexes
Time of Update: 2021-02-13
Two classes of histone acetyltransferases (HATs) have been described; cytoplasmic type B HATs acetylate free histones for subsequent assembly into chromatin ( 2 ) and nuclear type A HATs, which mediate transcription related acetylation of chromosomal histones ( 3 ).
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Spectrophotometric Determination of Histamine in Fisheries Using an Enzyme Immunoassay Method
Time of Update: 2021-02-13
The biogenic amines are low-weight organic bases that exhibit variable biological activity.
Approximately 30 vasoactive and psycoactive amines have been found in foodstuffs ( 1 ).
These amines occur naturally in animals, plants, and microorganisms owing to their natural metabolic processes, and are usually formed by decarboxylation of amino acids.
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鳡
Time of Update: 2021-02-13
It is a special fish of Heilongjiang, Yellow River, Yangtze River, Pearl River and so on in eastern China.
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Expression of Foreign Genes in Acholeplasma laidlawii
Time of Update: 2021-02-13
The expression of foreign genes in mollicutes has been difficult owing to inefficient transformation systems, and, until recently, by the lack of suitable cloning vectors.
Few genes, excluding those associated with vector functions, have successfully been cloned and expressed in mollicutes (see Table 1 ; 1 –8 ).
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Bacterial Artificial Chromosome Engineering
Time of Update: 2021-02-13
For example, a drug resistance marker may have to be inserted to enable selection of the BAC DNA when transfected into eukaryotic cells, a gene present in the BAC insert may have to be mutated for functional studies or a transgene may have to be introduced for ectopic expression.
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Assembly of DNA Sequencing Data
Time of Update: 2021-02-13
At this writing, public databases contain the completed, contiguous sequences of a large number of bacterial genomes (e.g., ) ( 1 ), the yeastSaccharomyces cerevisiae genome ( 2 ); and the genomes of the nematode ( 3 ), Drosophila ( 4 ), and Arabidopsis ( 5 ).
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Cutlass
Time of Update: 2021-02-13
For ferocious carnivorous fish, intense feeding is performed almost all the time except for spawning and low winter intake.
Fish from the northern wintering ground migrated from the wintering ground to the western coast in early 4, and 6 arrived at The Pishan and Da Chen near-shore spawning , after spawning scattered in the nearby sea area.
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Successful Culture Techniques for Helicobacter Species: General Culture Techniques for Helicobacter pylori
Time of Update: 2021-02-13
Half of the world’s population is persistently infected withHelicobacter pylori .
Thus, the ability to culture bacterial species from this genus is an extremely important step in expanding clinical knowledge and development of therapies.
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Isolation and Propagation of Mouse Liver-Derived Dendritic Cells
Time of Update: 2021-02-13
In the presence of GM-CSF, DC develop directly from myeloid committed precursors, which also give rise to monocytes and granulocytes, or from myelomonocytic cells, which are also precursors of monocytes ( 4 , 5 ).
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Quality Assessment of Finished BAC Sequences
Time of Update: 2021-02-13
However, for this chapter, we refer to finished sequence to mean the complete BAC clone from end-to-end finished to high quality and meeting or exceeding the finished sequence standards set by the publicly funded Human Genome Project consortium.
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An Optical Immunosensor for Pesticide Determination in Natural Waters
Time of Update: 2021-02-13
The river analyzer (RIANA) is an optical immunosensor able to determine different types of organic pollutants in water samples.
The RIANA integrates the optical part with a fluidics part in a compact unit, which is coupled to an autosampler, allowing the performance of automatic measurements within 15 min.
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Meat Protein Degradation by Tissue and Lactic Acid Bacteria Enzymes
Time of Update: 2021-02-13
Free amino acids are also the origin of other aroma volatile compounds that are involved in further enzymatic and chemical reactions ( 2 , 2 ).
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Selection of Functional Antibodies on the Basis of Valency
Time of Update: 2021-02-13
Often dimerization is a prerequisite for activation of a number of surface receptors by their natural ligands and divalent Abs are typically required for mimicking or blocking the activity of such ligands.
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Detection of Infectious Rotaviruses by Flow Cytometry
Time of Update: 2021-02-13
However, the sensitivity of these procedures is not high enough to detect the low number of viral particles sometimes present in the environment ( 3 ).
