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Half of the world’s population is persistently infected with
Helicobacter pylori
. The chronicity of this infection ultimately elicits clinical manifestations ranging from gastritis and peptic ulcers to adenocarcinoma and MALT lymphoma. Laboratory research following the initial observations of
Helicobacter
species was greatly hindered by an inability to isolate and culture the bacteria. Thus, the ability to culture bacterial species from this genus is an extremely important step in expanding clinical knowledge and development of therapies. This chapter describes successful techniques for culturing
H. pylori
on selective horse blood agar media and in Brucella broth liquid media. Additionally, the specific growth requirements of other
Helicobacter
species are noted.