A new method promotes the progress of pesticide residue detection technology and establishes a safety barrier for agricultural products

This detection method is based on the enzymatic cascade reaction ( HELP reaction) of synthesizing fluorescein analogs from phenolic compounds proposed by the research team
.
Organophosphorus pesticides and their metabolites are decomposed into phenol derivatives under the action of an enzyme from soil bacteria, and then through the HELP reaction to generate luciferin analogs, which react with luciferase to generate bioluminescent signals
.
As long as the fluorescence wavelength is analyzed, different organophosphorus pesticide species can be detected
.

 

Organophosphorus pesticides are widely used and the demand for detection is growing rapidly
 

In current agricultural production, the use of pesticides is the most common method to control pests and increase crop yields
.
Among them, organophosphorus pesticides (OPs) have become the main pesticide products due to their advantages of low cost, quick effect and wide range of targets
.

 

Although organophosphorus pesticides play an important role in controlling insect pests and ensuring food production, their harm to the environment and human body is gradually becoming known
.
Excessive pesticide residues in the environment may lead to soil and water pollution, ecological destruction, biodiversity reduction, and soil fertility decline
.
Organophosphorus pesticide residues in soil, water, agricultural products and other media can also lead to various health problems, including nervous system damage, behavioral disorders, immune system suppression, etc.
, and may cause serious cancer and other serious diseases.
disease
.

 

After the improvement of material living standards, people pay more attention to food quality and safety, and pesticide residues, as one of the main problems of agricultural products, have received a lot of attention
.
In recent years, China's management of pesticide use has become more and more strict, some highly toxic organophosphorus pesticides have been withdrawn from the market, and the pesticide residue standard system has been gradually improved
.
The demand for pesticide testing is developing rapidly in this context
.

 

　　The ease of use of detection methods remains to be improved
 

　　There are many methods for the detection of organophosphorus pesticides, including chromatography, spectroscopy, capillary electrophoresis, and biosensors
.
The current standard for the determination of organophosphorus pesticides and pesticides mainly uses chromatography, such as HJ 768-2015 "Gas Chromatography for the Determination of Organophosphorus Pesticides in Solid Waste", SN/T 3768-2014 "Multiple Organophosphorus Pesticide Residues in Exported Grains" Determination Methods Gas Chromatography-Mass Spectrometry", HJ 1189-2021 "Determination of 28 Organophosphorus Pesticides in Water Quality by Gas Chromatography-Mass Spectrometry",
etc.
In addition to gas chromatography, liquid chromatography and capillary chromatography can also be used for the detection of organophosphorus pesticides
.

 

　　Although chromatography has the advantages of low detection limit and high sensitivity, it needs to be carried out in the laboratory.
It is difficult to operate, takes a long time, and has high cost.
It is greatly limited in field application and rapid identification
.
Other detection methods also have their own limitations
.
At present, various detection technologies can meet the detection of pesticide residues in most cases, but the requirements for pesticide residue detection are constantly improving.
Small-volume detectors that are more convenient and cheap while maintaining high sensitivity and precision are urgent for the development of pesticide detection technology.
demand
.

 

　　Emergence of new methods to promote the development of pesticide detection technology
 

　　In response to market demand, new methods for the detection of organophosphorus pesticides continue to emerge, and the detection method based on the HELP reaction described above is one of them
.
China has also developed a new detection method for organophosphorus pesticides
.
In 2021, the quality and safety research group of the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences has successfully prepared a new type of porous composite material, which can be applied to the analysis of vegetable organophosphorus pesticide residues
.
Combined with solid-phase extraction technology, the method has the characteristics of high sensitivity, strong selectivity and good reproducibility
.
There are also portable sensors such as colorimetric sensors, fluorescent sensors, molecularly imprinted polymer-based sensors, and surface plasmon resonance-based sensors, which make pesticide detection more convenient
.

 

　　In recent years, the amount of pesticides used in crops across the country has been continuously reduced, and the utilization rate of pesticides has increased
.
At the same time, China is also eliminating low-efficiency, high-toxicity and high-residue varieties and promoting green pesticides and biological pesticides
.
Maybe one day, with the development of agricultural technology, organophosphorus pesticides will disappear in the market like DDT, 666 and other organochlorine pesticides in the past, but even so, we still have to close the inspection and supervision of pesticides and protect the ecological environment.
and people's health
.