echemi logo
  • Product
  • Supplier
  • Inquiry
    Home > Chemicals Industry > Chemical Technology > Abamectin drug residue analysis technology-extraction method

    Abamectin drug residue analysis technology-extraction method

    • Last Update: 2021-10-22
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit

    4 Residue analysis technology

    1 Pre-processing method

    Animal-derived food matrices mainly include animal tissues, milk, aquatic products, etc.
    , with complex components.
    Before the analysis and determination of residual AVMs, sample pretreatment is generally required to enrich trace AVMs from the complex matrix

    (1) Extraction method

    Before sample extraction, sufficient homogenization must be carried out to ensure the representativeness of the sample

    When adding a solvent for extraction, most of them use a homogenizer for extraction to ensure extraction efficiency

    1) Liquid liquid extraction (LLE)

    In view of the high fat solubility of AVMs, a variety of organic solvents can be used for extraction.
    Water-soluble organic solvents such as acetonitrile and methanol have better miscibility with the matrix; ethyl acetate and isooctane are also used

    Yang Junhong et al.
    used acetonitrile shaking to extract AVM, IVM, and EPR from bovine muscle tissue, and determined by an enzyme-linked immunoassay (ELISA) kit

    When the blank cattle muscle tissue was added at 5ng/g, 10ng/g and 20ng/g concentrations, the recovery rate of AVMs was 70.

    Cheng Linli and others extracted residual AVM, IVM, DOR and EPR in milk with acetonitrile, diluted with water and trace triethylamine , purified by solid-phase extraction column, dried with nitrogen and derivatized, and then measured by HPLC

    In the range of 2~1000ug/kg, the recovery rate of each drug is 70.

    Zhang Wenjuan and others established 10 methods for the analysis of residues of AVM, IVM, DOR, EPR, MOX and SEL in food matrices

    It was extracted with acetonitrile, purified by solid phase extraction, and determined by UPLC-MS/MS

    Yang Junhong et al.
    used methanol shaking to extract AVM, IVM and EPR from beef liver, and determined by ELISA kit

    When adding 20ng/g, 50ng/g and 100ng/g to the blank bovine liver tissue, the recovery rate of AVMs was 53.

    Xu Yingjiang and others studied the analysis methods of IVM, AVM, EPR and DPR in aquatic products

    The sample was extracted with isooctane, purified and derivatized by liquid-liquid extraction, and then detected by HPLC

    2) Pressurized liquid extraction (PLE)

    PLE refers to a new sample pretreatment method that uses solvents to extract solid or semi-solid samples at higher temperatures (50 to 200°C) and pressures (1000 to 3000 psi or 10.
    3 to 20.
    6 MPa)

    Compared with traditional extraction methods, PLE is fast, with less solvent consumption, high extraction efficiency, and good recovery rate of the components to be tested, which can realize fully automatic and safe operation

    3) Dispersive liquid liquid microextraction (DLLME)

    DLLME is a new type of micro-extraction technology that came out in 2006

    It is based on the use of a micro syringe to quickly inject the micro-upgrading extractant into the sample solution, forming extractant beads in the dispersant-water phase, which greatly expands the contact surface between the organic extractant and the water sample, and greatly accelerates the extraction balance The speed enables the target compound to be quickly extracted into the extractant microbeads, which improves the extraction efficiency and enrichment times

    4) Supercritical fluid extraction (SFE)

    SFE is a new type of material separation technology developed in the late 1970s.
    It is a method of extracting using fluid in a supercritical state as an extractant.
    Compared with traditional extraction methods, the separation efficiency is high.
    It can be changed by changing the temperature, Pressure or adding a small amount of organic solvent to optimize the extraction process and improve the extraction efficiency
    The supercritical fluid CO 2 is relatively frequently used, and has the characteristics of low toxicity, low cost, easy handling, good chemical stability, and easy to reach critical parameters (critical temperature of 31°C, critical pressure of 74 bar) and so on
    SFE can complete extraction and separation at the same time, with high separation efficiency, short operation cycle, fast mass transfer speed, strong dissolving power, high selectivity, and does not pollute the environment
    Danaher et al.
    reported the use of SFE technology to extract and purify the analysis method of AVM, IVM, EPR, DOR and MOX residues in animal livers
    Mix 2.
    5 g of homogenized sample with 4 g of diatomaceous earth, dehydrate and dry, and put it into an extraction cell sealed with polypropylene cotton and 2 g of basic alumina at both ends
    Extraction with supercritical CO 2 at 100℃ and 300bar , flow rate 5L/min, AVMs are adsorbed by the online alkaline alumina trap, and then eluted with 4mL formic acid-ethyl acetate (70+30, v/v), 60℃ Concentrate under nitrogen, after derivatization, determine by HPLC
    At the concentration of 4μg/kg and 20μg/kg, the average recovery rate of this method was 76% and 97%, and the intra-day and inter-day RSD were less than 10% and 16%, respectively; LOQ reached 2ug/kg
    This method has also been used for the analysis of pig liver and sheep liver
    When Brooks et al.
    used the SFE method to extract AVM from animal tissues, a 9% modifier-ethylene glycol methyl ether (2-methoxyethanal, EGMME) was added to the supercritical fluid CO 2 to improve the extraction efficiency
    At a concentration of 22ng/g, no interferences were found

    Related link: Maximum allowable residue limits of abamectin drugs



    This article is an English version of an article which is originally in the Chinese language on and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to with relevant evidence.