Applicability of the method for determination of 8 macrolides and lincosamide residues in royal jelly and royal jelly freeze-dried powder

6.
2.
3.
10 Applicability of the method

Using this method, the eight antibiotics in royal jelly were added at four levels of 1 LOD (2.
0μg/kg), 2 LOD (4.
0μg/kg), 4 LOD (8.
0ug/kg), and 10 LOD (20.
0μg/kg).
, Freeze-dried powder in 1 LOD ( lincomycin , erythromycin , clindamycin , tylosin and josamycin are 2.
0μg/kg, tilmicosin, spiramycin, kititamycin 5.
0μg/kg), 2LOD (lincomycin, erythromycin, clindamycin, tylosin and josamycin are all 4.
0μg/kg, tilmicosin, spiramycin, guitar Both lincomycin, erythromycin, clindamycin, tylosin and josamycin are all 8.
0 μg/kg), 4LOD (lincomycin, erythromycin, clindamycin, tylosin and josamycin are all 8.
0μg/kg, tilmicosin, spiramycin , Kitasamycin are 20.
0μg/kg), 10LOD (lincomycin, erythromycin, clindamycin, tylosin and josamycin are all 20μg/kg, Tilmicosin, Spirulina ( Both 50μg/kg) for four addition levels of kitasamycin and kitasamycin), the recovery rate experiment of adding 8 kinds of antibiotics was carried out, and the analysis results are shown in Table 6-27 and Table 6-28
.

Table 6-27 Applicability of the method Adding recovery rate (%, n=10)

Table 6-28 Experimental data of suitability of 8 antibiotics in freeze-dried powder (%)

6.
2.
3.
11 Repeatability and reproducibility

Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
.

Table 6-29 Content range and repeatability and reproducibility equation