Bamboo chrysanthemum A

Bamboo chrysanthemum A

Raddeanin A

【Structural formula】

[Molecular formula and molecular weight] C ₄₇ H ₇₆ O ₁₆ ; 897.

[Source] The rhizome of Anemone raddeana Regel, an anemone of the Ranunculaceae family

【Properties】Needle crystals

This product is soluble in water

Melting point: 242～244℃

Optical rotation: [α] _D ²⁵ —8°(CH ₃ OH)

【Purity Check】

TLC

1.

Eluent petroleum ether - ethyl acetate - methanol - acetic acid (8:5:0.

Check vanillin sulfuric acid color development, check under sunlight

2.

Developing agent chloroform- acetone - n-hexane - glacial acetic acid (9:4:0.

Check vanillin sulfuric acid color development, check under sunlight

High performance liquid chromatography

Column Agilent Zorbax C ₁₈ , 5um (4.

The mobile phase methanol -0.

Detection wavelength 210nm

【Structural identification】203

3100～3600, 2900, 1697, 1640, 1465, 1398, 1365, 1310, 1230, 1154, 1120, 1070, 1050, 1024, 885, 845, 810, 710

FD-MS m/z: 919 [M+Na] ⁺

¹ H-NMR (C ₅ D ₅ N) δ: 0.
85, 1.
32 (each 3H, s, 2×CH ₃ ), 5.
48 (1H, t, J=3.
40 Hz, H-12), 4.
82 (1H, d, J=6.
0Hz, H-1'), 5.
20 (1H, d, J=6.
0Hz, H-1''), 6.
16 (1H, d, J=8.
0Hz, H-1'''), 1.
67 ( 3H, d, J=6.
5Hz, H-6''')
.

¹³ C-NMR (C ₅ D ₅ N) δ: 39.
0(C-1), 26.
2(C-2), 88.
8(C-3), 39.
5(C-4), 56.
0(C-5), 18.
7( C-6), 33.
3(C-7), 39.
8(C-8), 48.
1(C-9), 37.
0(C-10), 23.
8(C-11), 122.
6(C-12), 145.
0(C -13), 42.
2(C-14), 28.
3(C-15), 23.
8(C-16), 46.
7(C-17), 42.
2(C-18), 46.
7(C-19), 31.
0(C- 20), 34.
2(C-21), 33.
3(C-22), 28.
1(C-23), 17.
1(C-24), 15.
5(C-25), 17.
4(C-26), 26.
2(C-27) ), 180.
2(C-28), 33.
3(C-29), 23.
8(C-30), 104.
9(C-1'), 76.
4(C-2'), 72.
5(C-3'), 69.
8(C -4'), 64.
5(C-5'), 106.
3(C-1''), 79.
6(C-2''), 75.
4(C-3''), 71.
4(C-4''), 78.
6 (C-5''), 62.
5(C-6''), 101.
8(C-1'''), 72.
5(C-2'''), 72.
1(C-3'''), 74.
0(C -4'''), 69.
8(C-5'''), 18.
7(C-6''')
.

【Storage】Dry and airtight
.