Codonopsis

Codonopsis

Lobetyolin

【Structural formula】

[Molecular formula and molecular weight] C ₂₀ H ₂₈ O ₈ ; 396.

[Source] The dried root of Codonopsis pilosula (Franch.

【Properties】Brown-yellow block crystals

This product is easily soluble in methanol , ethanol , acetone and ethyl acetate , soluble in ether, acetic acid and chloroform , slightly soluble in benzene and water, and insoluble in carbon disulfide and petroleum ether

Melting point: 70～72℃

【Purity Check】

TLC

1.

Developing solvent n-butanol - acetic acid - water (7: 1: 0.

After checking the color development of 10% sulfuric acid ethanol solution, check under sunlight and ultraviolet light (365nm)

2.

Developing agent chloroform-methanol- glacial acetic acid (9:1:0.

After checking the color development of 10% sulfuric acid ethanol solution, check under sunlight and ultraviolet light (365nm)

High performance liquid chromatography

Column Phenomenex Gemini C ₁₈ 110A, 5um (4.

Mobile phase 0-30min, acetonitrile -water (80:20); 30-60min, acetonitrile-water (80:20)→(20:80), 1ml/min

Detection wavelength 267nm

【Structural identification】283,268,253,241,215,207

3385 (-OH), 2924, 2879, 2235 (C=C), 1626 (C=C), 1439, 1078, 1043, 949

ESI-MS m/z: 831[2M+K] ⁺ , 815[2M+Na] ⁺ , 435[M+K] ⁺ , 419[M+Na] ⁺

¹ H-NMR (CD ₃ COCD ₃ ) δ: 1.

¹³ C-NMR (CD ₃ COCD ₃ ) δ: 18.

【Storage】Dry and airtight
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