Construction of a new pathway for vitamin K2 biosynthesis in Pichia pastoris

Zheng Zhiming, research team of Institute of technology, biology and agricultural engineering, Hefei Institute of material science, Chinese Academy of Sciences, applied the concept of synthetic biology to construct a new pathway of vitamin K2 biosynthesis in Pichia pastoris The relevant research results were published in microbial cell factories (DOI: 10.1186 / s12934-019-1215-9) Based on human aromatic isopentenyltransferase (hsubiad1), the research group constructed rDNA mediated multi copy expression vector to realize the fusion expression of saggpps and ppidi, further strengthen the supply of isopentenyl pyrophosphate side chain, and promote the synthesis of MK-4 This study is the first to realize the whole cell catalytic synthesis of MK-4 in Pichia pastoris, which lays a foundation for the rational design and pathway construction of menadione isoprene derivative cells, and has guiding significance for the biosynthesis of high value chemicals The research work was supported by the national high-tech development research plan and major science and technology projects of Anhui Province.