Detection of hypochlorite in cells by Chen Xueyuan's team of Fujian Institute of physical architecture based on double excitation ratio rare earth up conversion fluorescence labeling

The changes of cell microenvironment are closely related to many physiological and pathological processes The development of non-invasive fluorescent probes to monitor the tiny changes of biological molecular content or physiological parameters in cells has important biological significance and medical value However, most of the current intracellular fluorescence analysis methods only provide non quantitative fluorescence imaging, and its sensitivity and accuracy are difficult to meet the actual monitoring needs Chen Xueyuan, a Key Laboratory of functional nanostructure design and assembly, Fujian Institute of physical architecture, Chinese Academy of Sciences, supported by the special project of strategic leading science and technology of Chinese Academy of Sciences, the international innovation team of Chinese Academy of Sciences, the National Natural Science Foundation of China and the youth Promotion Association of Chinese Academy of Sciences chaired by Lu Shan, a deputy researcher, For the first time, the ratio up conversion strategy of near-infrared double excitation was proposed to realize the accurate detection of intracellular biomolecules The team developed a polymer F127 coated water-soluble dye-sensitized rare earth upconversion fluorescence probe, which uses the high-efficiency energy transfer and low background fluorescence signal of near-infrared dye ir808 to Yb / ER Co doped nagdf 4 upconversion nanoparticles to realize the ultra sensitive detection of hypochlorite (ClO -) under the excitation of 808 nm, with the detection limit as low as 16.1 nm At the same time, the up conversion luminescence excited by 980 nm was used as the reference signal in this study, which reduced the detection deviation caused by the complex environment and uneven probe distribution Through the design of a new near-infrared double excitation confocal microscope system, the research team successfully achieved the accurate quantitative analysis of endogenous and exogenous ClO - in living cells MCF-7 By changing the response groups of NIR dyes, the double excitation ratio detection strategy can be extended to the detection of various intracellular biomolecules, providing an important tool for the monitoring of physiological processes of living cells and disease diagnosis The research results were published in adv SCI (DOI: 10.1002 / advs 201901874) under the title of "a strategy of NIR dual ‐ exceptionupconversion for quantitative internal detection" The first author of this paper is Ke Jianxi, Ph.D candidate of lianpei, Fujian Institute of physical architecture / Shanghai University of science and technology.