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(7) Liquid chromatography-mass spectrometry (LC-MS)
LC-MS has been widely used in the analysis of non-steroidal anabolic hormone residues.
It is currently the most sensitive analysis and detection method.
It can also provide: compound structure information without derivatization, but the equipment is expensive, which limits the scope of application
.
Zhu Yong et al.
established an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analytical method for the simultaneous determination of DES and other drug residues in milk
.
The milk sample was directly purified by the HLB cartridge, washed with water and n-hexane , and eluted with ethyl acetate for analysis
.
Peng Tao et al.
established an LC-MS/MS method to detect TAL, a-ZOL, β-ZOL, ZER, ZAN and ZON in animal liver
.
The liver samples were hydrolyzed by enzymes, extracted with ether, purified by HLB solid phase extraction column, and detected by LC-MS/MS
.
Kathrin et al.
established an LC-MS/MS method to simultaneously detect stilbene compounds (DES, DIS, HES) and RALs (ZER, TAL, a-ZOL, β-ZOL and ZON) in cow urine
.
Urine samples were hydrolyzed with enzymes, extracted with ether, purified by SPE, and analyzed by LC-MS/MS
.