Determination method of sulfa drug residues (4)

4) Electrochemical detector (ECD)

ECD is a sensitive and cheap detection method.
HPLC-ECD is an order of magnitude more sensitive to the determination of SAs residues than DAD.
However, because SAs contaminate the electrode and inactivate it, it is necessary to pre-process the electrode or use pulsed cyclic potential scanning.
Regeneration electrode
.
Wang Jianhua and others used HPLC-ECD method to determine the residual amount of three kinds of SAs in chicken

Rao et al.
reported a quick and easy HPLC-ECD method for the determination of SAs
.
Conductive boron-doped diamond film electrodes are used for electrochemical analysis of sulfadiazine, sulfamethazine and sulfamethazine

5) Chemiluminescence detector (CLD)

The HPLC-CID developed in recent years is a promising high-sensitivity detection method.
It uses bis(2,2) trichlorophenyl oxalate (TCPO) to interact with hydrogen peroxide to excite a phosphor to emit light
.
The luminescence value of the chemiluminescence reaction is related to factors such as the concentration and ratio of the reactants, the reaction medium and the length of the mixing tube

Soto-Chinchilla et al.
used HPLC-CID for the first time to detect 7 SAs in milk
.
After the analytes are separated by HPLC, they are derivatized with fluorescamine under the catalysis of imidazole in a chemiluminescence system

Related Links: Determination of Sulfonamide Drug Residues (3)