Determination of 9 penicillin residues in puffer fish and eels-linear range and lower limit of determination

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8 Linearity range and lower limit of determination

The preparation contains piperacillin , penicillin G, penicillin V, oxacillin , and nafcillin at concentrations of 1.
25 to 100 ng/mL (0.
5 to 40 LOD); the concentrations of amoxicillin , ampicillin , cloxacillin, and dicloxacillin are respectively 2.
5～200ng/mL (0.
5～40LOD) sample matrix mixed standard solution, measured under selected conditions, the injection volume is 20uL, and the peak area is used to plot the concentration of each component in the standard solution.
The absolute amounts of piracillin, penicillin G, penicillin V, oxacillin, and nafcillin are in the range of 0.
025-2ng respectively: the absolute amounts of amoxicillin, ampicillin, cloxacillin, and dicloxacillin are in the range of 0.
05-4ng respectively All have a linear relationship and meet the quantitative requirements.
The linear equations and correction factors are shown in Table 5-23
.

Table 5-23 Linear equations and correction factors of 9 penicillins

Under the operating conditions of this method, when the added level of 9 penicillins is 1.

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9 Method recovery and precision

Use penicillin-free puffer fish and eel samples for addition recovery and precision experiments.
After adding four different levels of 9 penicillin standards at 1.
0～20μg/kg (LOD～10 LOD) to the samples, shake them well to make the standards It is fully absorbed with the sample, and then extracted and purified according to this method.
The recovery rate and precision are shown in Table 5-24
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Table 5-24 The recovery rate of 9 penicillins in puffer fish and eels (%) (n=10)

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Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
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Table 5-25 The content range of 9 penicillins and the reproducibility limit and reproducibility limit equation

Related Links: Determination of 9 Penicillin Residues in Puffer Fish and Eels-Selection of Analysis Conditions