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(6) High performance liquid chromatography (HPLC)
The molecular structure of CAPs contains a non-free ammonia, which is a moderately polar compound and can be separated by reversed-phase liquid chromatography (RP-LC)
.
Non-polar alkyl bonded phase is currently the most widely used column packing.
1) UVD and DAD/PDA
Li et al.
established an HPLC-PDA method for the determination of CAP in pork and liver
.
The recovery rates of pork and pig liver were 91.
In recent years, the research on FF residues at home and abroad has mostly adopted the HPLC method reported by Hormazabal and others
.
Extract muscle tissue or liver with water and acetone, centrifuge to remove the lower residue, and purify the supernatant with dichloromethane LLP, discard the water layer, concentrate to dryness, add 0.
Nagata et al.
reported the determination of TAP, FF and CAP in muscle and fish by HPLC
.
Extract with ethyl acetate.
2) FLD
Xie et al.
used RP-LC-FLD to detect TAP, FF and FFa in eggs
.
After ultrasonic extraction with 1 mL of acetonitrile-water (30+70, v/v) and 20 mL of ethyl acetate-acetonitrile-ammonia (49+49+2, v/v) for 5 min, blow dry with nitrogen, reconstitute acetonitrile, and n-hexane Degreasing, C18 chromatographic column separation, HPLC-FLD detection
The CAP structure contains aromatic nitro groups, which can be derivatized with fluorescein, and amino groups are added to produce fluorescence, which can be detected by FLD
.
Pan Yingyu and others established an HPLC-FLD detection method to detect the residual amount of CAP in milk
Table 4-4 HPLC detection methods for CAPs residues in some animal-derived foods
Related Links: Determination of Chloramphenicol Residues-Thin Layer Chromatography and Capillary Electrophoresis
