Determination of Hesperidin in Zhishidaozhi Pills and Total Saponins in Ginseng Injection

Determination of Hesperidin in Zhishidaozhi Pills

[Prescription] Citrus aurantium (stir-fried) 100g Rhubarb 200g Coptis (made from ginger juice) 60g

Scutellaria 200g Liu Shenqu (fried) 100g Atractylodes (fried) 100g Poria 60g Alisma 40g

[Content determination] Preparation of standard curve: Precisely draw 1~5ul of hesperidin reference solution, dot them on the same polyamide film, use methanol as the developing agent, expand as above, develop color, and scan according to the above conditions

In the formula, a is the intercept of the ordinate; b is the slope or proportionality constant of the straight line; X is the weight or concentration of the component

Correlation coefficient: r=0.

Preparation of the test solution: Take an appropriate amount of this product, grind it, take about 0.

Preparation of reference substance solution: Take another appropriate amount of hesperidin reference substance, accurately weigh it, and add methanol to make a solution containing 50pg per 1ml as a reference substance solution

Measurement conditions: CS-930 thin-layer scanner, MPE-4 fluorescence spectrophotometer, fluorescence linear scanning

Determination method: Precisely draw 5ul of the test solution, 2ul and 5ul of the reference solution, and intersect them on the same polyamide thin mold respectively.

Each 1g of this product contains Citrus aurantium as hesperidin (C ₂₈ H ₃₄ O ₁₅ ), not less than 20.

Determination of total saponins in ginseng injection

This product is a sterile aqueous solution made by ethanol extraction of ginseng (Panax ginseng CAMey)

Preparation of the control solution: Take an appropriate amount of the total ginsenoside reference substance, accurately weigh it, and use methanol to make a solution containing about 0.

Preparation of the test solution: accurately measure 10ml of this product, place it in a 100ml separatory funnel, degrease with ether twice, 20ml each time, extract the water layer with water-saturated n-butanol 5 times, 18ml each time, combine the extracts , Wash twice with n-butanol saturated water, 20ml each time, divide the n-butanol extract, put it in a 100ml measuring flask, add water-saturated n-butanol to dilute to the mark, shake well, and get it

In the formula, A1 is the absorbance of the control solution; A2 is the absorbance of the test solution; C1 is the concentration of the control solution (mg/ml); P is the purity of the total ginsenoside reference substance