Determination of linear range and lower limit of 8 macrolides and lincosamide drugs in honey

6.
2.
2.
8 Linearity range and lower limit of determination

Prepare mixed standard solutions with concentrations of 2.
0ng/mL, 5.
0ng/mL, 10.
0ng/mL, and 50.
0ng/mL, and measure them under selected conditions.
The injection volume is 20μL.
Plot the concentration of each tested component
.

Table 6-20 Linear equations and correlation coefficients

Under the operating conditions of this method, when the 8 antibiotics are added at a level of 2.
0-50μg/kg, the response value is within the linear range of the instrument, and the signal-to-noise ratio S/N of the measured spectrum is greater than 5
.

6.
2.
2.
9 Method recovery and precision

Use honey samples that do not contain the above 8 antibiotics for addition recovery and precision experiments.
After adding 2.
0μg/kg, 5.
0μg/kg, 10.
0ug/kg, and 50.
0μg/kg different concentration standards to the sample, shake it well to make the sample Fully absorb, and then perform extraction, purification, and determination according to this method.
The recovery rate and precision are shown in Table 6-21
.

Table 6-21 Adding recovery rate and precision experimental data (n=10)

Related Links: Selection of Analysis Conditions for 8 Macrolides and Lincosamide Drugs in Honey