Determination of Loganin in Liuwei Dihuang Pills

Determination of Loganin in Liuwei Dihuang Pills

[Content determination] Jujube in wine: According to the 2015 edition of "Chinese Pharmacopoeia" four parts of high performance liquid chromatography (General Principle 0512) determination

Chromatographic column: Zorbax SB C ₁₈ , 4.

Flow rate: 1.

Instrument configuration: quaternary pump with vacuum deaerator (G1354A)

Autosampler (G1367A)

Column thermostat (G1316A)

UV detector (G1314A)

Instrument control and data processing: Agilent Chemstation

Preparation of reference substance solution: Take an appropriate amount of loganin reference substance, accurately weigh it, add 50% methanol to make a solution containing 20ug per 1ml, and get it

Preparation of the test solution: take this product water honey pill or small honey pill, chop it, take about 0.

Note: The injection volume is 5ul

(100~200 mesh, 4g, inner diameter 1cm), elute with 40% methanol 50ml, collect the effluent and eluate, evaporate to dryness, add 50% methanol to the residue to dissolve, and transfer to a 10ml volumetric flask, Dilute with 50% methanol to the mark, shake well, and get it