Determination of Purity of Ursolic Acid—High Performance Liquid Chromatography (1)

1 Scope

This standard specifies the test method for determining the purity of ursolic acid by high performance liquid chromatography

This standard applies to the determination of the mass fraction of ursolic acid in ursolic acid products extracted from loquat leaves, rosemary and other plants

2 Normative references

The clauses in the following documents constitute the clauses of this standard through the reference of this standard

GB/T6682-2008 Analytical laboratory water specifications and test methods (ISO 3696:1987, MOD)

3 Test method

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Some test procedures specified in the test method may lead to dangerous situations, and the operator should take appropriate safety and protective measures

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Use high performance liquid chromatography, under selected working conditions, separate the target compound and impurities in the sample solution through a chromatographic column, detect with an ultraviolet absorption detector, and quantify with an external standard method to calculate the mass fraction of ursolic acid in the sample

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4 Data processing system: chromatographic workstation or data processor
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2 Suction filtration system

The suction filtration system uses a cellulose ester filter membrane with a pore size of 0.
45um (used for pretreatment of the mobile phase)
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3 Filter system

The filtration system uses a cellulose ester filter membrane with a pore size of 0.
45um (used for sample pretreatment)
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4 Sampler

Autosampler, or micro-injection needle (for HPLC): 50uL
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Related link: Determination of relative correction factor