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(6) High performance liquid chromatography (HPLC)
The pyrazolone structure of this class of drugs has ultraviolet absorption characteristics, and HPLC analysis mainly adopts reversed-phase chromatographic separation and ultraviolet detector (UVD) determination
.
1) Acidic drugs
Pang Guofang et al.
used reversed-phase HPLC to determine phenylbutazone in animal muscles
.
Use 0.
Neto et al.
used HPLC-UVD to determine phenylbutazone and oxybutazone in blood
.
The purified sample was separated by a Chrospher RP-18 column, the mobile phase was 0.
Haque et al.
injected plasma directly into the HPLC system to determine phenylbutazone and oxybutazone
.
The semi-permeable surface column 5PM-S5-100-C 18 (15 cm×4.
2) Basic drugs
Katz et al.
established an HPLC method for the determination of four metabolites of Analgin in plasma
.
After the sample was alkalized with sodium hydroxide solution, it was extracted with dichloromethane , the extract was evaporated to dryness, the mobile phase was reconstituted and determined by HPLC
Shen Jincan and others established an HPLC method for the determination of the metabolites FAA, AA and MAA of Analgin in cattle and pig muscles
.
It is separated by Inertsil ODS3 chromatographic column, water and methanol are mobile phases, gradient elution, flow rate 1mL/min, detection wavelength 265nm
MikatimA et al.
established an HPLC method for the determination of antipyrine and its metabolites in urine
.
After the urine is enzymatically hydrolyzed, it is extracted with ethyl acetate, and the extract is evaporated to dryness and reconstituted with mobile phase and analyzed by HPLC
Related Links: Determination of Pyrazolone Drug Residues-Gas Chromatography-Mass Spectrometry