Determination of the content of active ingredients in traditional Chinese medicine (3)

2.

Because of the specific chemical structure of flavonoids, most of the compounds show color in visible light (or after adding a suitable coloring agent), and have strong absorption in the ultraviolet region.

Determination of total flavonoids: After a certain amount of extraction and separation of raw materials and preparations containing flavonoids, their absorbance can be measured directly at the maximum absorption wavelength

Thin-layer chromatography is one of the effective methods to determine monomer flavonoids in preparations

3.

Saponin complex chemical structure, which are commonly used determination method for gravimetric method, a neutralization method, thin layer chromatography, colorimetry, UV spectrophotometry, HPLC and the like

In Chinese patent medicine preparations, the quantitative analysis of saponins can be divided into total saponins determination, sapogenin determination and monomer saponins determination

The most commonly used method for the determination of total saponins is the colorimetric method, and the gravimetric method is also useful

The methods for determining the content of sapogenin mainly include thin layer chromatography, high performance liquid chromatography and colorimetry

1.

2.

4.

1.

2.

3.

TLC is a commonly used quantitative analysis method for anthraquinone components.

Related Links: Determination of Active Ingredients in Traditional Chinese Medicine (2)