Determination of Zearalenone in Cereals

This article introduces the immunoaffinity chromatography purification high performance liquid chromatography method to determine the content of zearalenone in cereals (according to GB/T5009.
209-2008)
.

1.
Principle

Zearalenone in the sample was extracted with acetonitrile -water, and the extract was purified and concentrated by an immunoaffinity column, and then determined by a liquid chromatograph equipped with a fluorescence detector, and quantified by an external standard method
.

In the formula, X——the content of zearalenone in the sample, ug/kg

A——The peak area of ​​zearalenone in the sample solution

A ₀ ——The peak area of zearalenone in the blank sample solution

p——The concentration of zearalenone in the standard working solution, ug/mL

V——The final constant volume of the sample solution, mL

m——The amount of sample represented by the final sample solution, g

As-the peak area of ​​zearalenone in the standard working solution

2.

Chromatographic column: C ₁₈ column, 150mm×4.
6mm (inner diameter), particle size 4um, or equivalent; mobile phase: acetonitrile -water- methanol (46+46+8); flow rate: 1.
0mL/min; detection wavelength: excitation wavelength 274nm, emission wavelength 440mm; injection volume: 100uL; column temperature: room temperature
.

Under the above chromatographic conditions, the retention time of zearalenone is 3.
4 min
.

3.
Tips

This method is applicable to the determination of zearalenone in grains (corn, wheat, etc.
)
.

Figure 6-9 Liquid chromatogram of zearalenone standard