Fluorescence Identification of Tianwang Buxin Pills, Amber Baolong Pills and Ginkgo Leaves

Fluorescence Identification of Tianwang Buxin Pills

[Prescription] Danshen 25g Angelica 50g Shichangpu 25g Codonopsis 25g Poria 25g Schisandra 50g Ophiopogon 50g Asparagus 50g Rehmannia 200g Scrophulariaceae 25g

[Identification] Take 1g of this product, mash the water honey pill; cut the small honey pill or honey pill, spread it flat in a crucible, cover with a funnel with a long handle, and heat slowly until the powder is slightly coked, stop heating, let it cool, and take Lower the funnel, rinse the inner wall with 5ml of water, and observe the lotion under an ultraviolet lamp (365nm), showing a light blue-green fluorescence (Salvia, Angelica, etc.

Fluorescence Identification of Amber Baolong Pills

[Prescription] yam (fried) 256g cinnabar 80g licorice 48g amber 24g geranium 24g sandalwood 24g citrus aurantium (fried) 16g poria cocos 24g dannan star 16g citrus fruit (fried) 16g red ginseng 24g

[Identification] Take 3 pellets of this product, cut into pieces, add 5g of diatomaceous earth, grind finely, add 80ml of methanol, heat to reflux for 2 hours, filter, evaporate the filtrate, add 30ml of water to the residue to dissolve, shake with chloroform to extract 2 times, 30ml each time, discard the chloroform solution, shake and extract the water solution 3 times with n-butanol, 30ml each time, combine the n-butanol solution, wash 2 times with ammonia test solution, 80ml each time, take the positive The butanol solution was evaporated to dryness, and the residue was dissolved by adding 1ml of methanol and used as the test solution

Fluorescence Identification of Ginkgo Leaves

This product is made of ginkgo biloba extract after processing

[Identification] (1) Ginkgo biloba extract total flavonol glycosides: take an appropriate amount of this product (approximately containing 48mg of total flavonol glycosides), remove the coating, grind, add 15ml n-butanol, and soak in a water bath for 15 minutes Shake it from time to time, let it cool, filter, evaporate the filtrate, add 2ml of ethanol to the residue to dissolve it, and use it as the test solution

(2) Terpenoid lactones:

1) Preparation of reference solution: Take appropriate amounts of bilobalide reference substance, ginkgolide A reference substance, ginkgolide B reference substance and ginkgolide C reference substance respectively, accurately weigh them, and add methanol to make each containing each 1ml 1mg, 1mg, 1mg, 0.

2) Preparation of test solution: Take 20 tablets of this product, remove the coating, accurately weigh, and grind, take the powder equivalent to 19.

Note: Ginkgo biloba extract (GBE) is extracted and refined from ginkgo biloba using advanced extraction technology