Get Insight into the SepaBean Gamma Machine with Engineer: Evaporative Light Scattering Detector

In the last special lecture on sepabean ® machine, engineers of application technology research center introduced the photodiode array detector (DAD) to you, focusing on the full band scanning function of dad detector in sepabean ® machine system of Santai technology In today's technical knowledge lecture, I'd like to introduce the latest general quality detector of Santai technology, the evaporation light scattering detector (ELSD) ELSD belongs to the quality detector Unlike UV or FL detector, the signal response of ELSD does not depend on the optical characteristics of the sample Any sample whose volatility is lower than the mobile phase can be detected without the influence of its functional groups The signal response value of ELSD is directly proportional to the mass of the sample, so it can be used to determine the purity of the sample or to detect the unknown Compared with ELSD, the differential detector (RI) is also a general-purpose detector, but its sensitivity is low and incompatible with gradient elution, which limits its application In addition, mass spectrometry (MS) is another universal detector, but its high cost and complex operation limit its wide application As a result, ELSD has become a detector favored by more and more researchers due to its advantages of high sensitivity, wide range of application, simple operation and relatively low cost It is widely used in the analysis and detection of sugars, phospholipids, amino acids, fatty acids, steroids, saponins and polymers without UV absorption Next, I'd like to introduce sepaflash, the ELSD module launched by Santai technology Gamma The detection principle of FP lt-elsd is mainly divided into the following three steps: the first step: after the eluate product of the atomizing chromatographic column is mixed with the inert gas, it is ejected through the narrow pinhole of the atomizer to form uniform droplets These fine droplets consist of mobile phase droplets containing the target compound The second step: the evaporated droplet passes through the heated drift tube, the mobile phase in the droplet is evaporated, and the solute forming very fine solid particles are retained and enter the flow detection pool The third step is to detect the flow of solute particles into the flow detection cell, which includes a light source and a photodiode The light emitted by the light source impacts the solute particles to scatter, and the scattered light is detected by the photodiode and converted into an electrical signal Taking the preparation and purification of a synthetic drug intermediate as an example, this paper introduces the application of ELSD in the actual sample purification The chemical structure formula of a drug intermediate is shown in Figure 1: Figure 1 Analysis of the chemical structure formula of a drug intermediate shows that the compound has no UV absorption, so the commonly used UV detector can not be used for real-time monitoring of the sample in the flash preparation and purification process, and the ELSD detector as a general-purpose detector can well complete the task The flash preparation conditions of the sample are shown in Table 1, and the flash preparation separation spectrum of the sample is shown in Figure 2 Table 1 Sample flash preparation experimental parameter instrument sepabean ® machine t chromatographic column 12g sepaflash ® standard series positive phase purification column (amorphous silica gel, 40-63 μ m, 60 μ m, order No.: s-5101-0012) detector UV: 254 nm; 280 nm sepaflash Gamma FP lt-elsd mobile phase solvent A: petroleum ether solvent B: flow rate of ethyl acetate 30 ml / min (ELSD shunt: 0.5 ml / min) sample volume 600 mg elution gradient column volume (CV) solvent B (%) 0.11 18 15 18 21 34 24 34 Figure 2 Based on innovative technology and professional services, Santai technology is committed to providing customers with complete separation and purification solutions To learn more about the detailed specifications of sepabean ® machine, or the ordering information of the flash column used with it, please visit CBG online store Previous special lecture engineer will take you to know the special lecture of SepaBean machine -- DAD detector