Ginkgolide A

Ginkgolide A

GinkgolideA

【Structural formula】

[Molecular formula and molecular weight] C ₂₀ H ₂₄ O ₉ ; 408.

【Source】Leaf and root bark of Ginkgo biloba L.

【Properties】White fine-grained crystals

This product is easily soluble in methanol and acetone , but insoluble in water

Melting point: It will turn yellowish brown and decompose above 300℃

【Purity Check】

TLC

1.

Developing solvent cyclohexane - acetone - methanol (2.

After 15 minutes of acetic anhydride vapor fumigation, inspect under UV light (365nm)

2.

Developing agent toluene- ethyl acetate - acetone - methanol (5:2.

After 15 minutes of acetic anhydride vapor fumigation, inspect under UV light (365nm)

High performance liquid chromatography

Chromatographic column Platinum C ₁₈ , 5um (4.

Mobile phase 0-15min, methanol-water (22:78), 15-50min, methanol -water (22:78)→(80:20), 1ml/min

Alltech 2000ES ELSD detector, nitrogen: 2.

【Structural identification】204

3494, 3255 (-OH), 1802, 1763 (C=O), 1361, 1330, 1138, 1045

EI-MS m/z: 408[M]+

¹ H-NMR (DMSO-d ₆ ) δ: 1.

¹³ C-NMR (DMSO) δ: 35.
9(C-1), 85.
1(C-2), 68.
0(C-3), 100.
2(C-4), 86.
0(C-5), 87.
6(C-6) , 36.
3(C-7), 48.
5(C-8), 66.
8(C-9), 68.
7(C-10), 174.
2(C-11), 109.
4(C-12), 170.
7(C-13), 40.
4 (C-14), 176.
5 (C-15), 8.
1 (C-16), 31.
9 (C-17), 28.
8 (C-18, 19, 20)
.

【Storage】Dry and airtight
.

Related link: kaempferol