JACS: the joint research center of felinga Nobel Prize scientists of East China University of science and technology to realize the "double detection" biological imaging of light controlled fluorescence

Recently, the Journal of American Chemical Society, a famous international journal in the field of chemistry, reported on-line the latest research results (DOI: 10.1021 / JACS 8b05425) of the joint research center of feilinga Nobel Prize scientists, School of chemistry and molecular engineering, East China University of science and technology, entitled "photo controlled fluorescence" double check "bioimaging enabled by aglycoprobe − protein hybrid" In recent years, cell and in vivo imaging technology has developed rapidly, but the design and synthesis of chemical probes that can be precisely located and remotely regulated in living cells is still a challenging interdisciplinary research topic In order to solve this key problem, the researchers combined gal NSP with HSA through the host guest interaction, and simply constructed a protein light-controlled fluorescence probe complex which can realize the precise positioning of double system fluorescent signal cells and target recognition through remote light control It is found that, unlike the traditional single mode photochromic probe, the compound probe system can realize the reversible regulation of spiropyran / part cyan structure and the switch between green and red fluorescence modes (i.e "on-off" of green fluorescence of fluorophore) by means of UV / visible light cycle“ "Off on" with red fluorescence of photochromic dye (Fig 1) Figure 1 Photoluminescence "double detection" for living cell imaging (photo source: J am Chem SOC.) researchers have studied the binding mode and action site between glycosyl probe and protein through small angle scattering (SAXS) technology, proving that probe molecules can cause the latter IIIA by combining with HSA Significant folding of the area near the water level point Furthermore, the constructed probe protein complex was applied to realize cell precise fluorescent labeling: firstly, the galactose group of the composite probe can specifically recognize and start endocytosis with ASGPR receptor on the membrane surface of Hep-G2 cell, and realize active cell localization; secondly, through the regulation of UV / visible light cycle, the probe can perform multiple repeatable in the cell“ The phenomenon of "double fluorescence flicker" can realize the reciprocating switch between green fluorescence and red fluorescence, thus improving the positioning accuracy of fluorescence probe in the complex cell environment, so as to achieve the purpose of double detection and precise positioning (Figure 2) On the one hand, this study provides a new photo controlled chemical probe tool for cell targeting and precise functional labeling research On the other hand, it proposes a universal design strategy to realize the dual fluorescence response of organic photochromic fluorescent probe in physiological environment through protein loading, which solves the problem of fluorescence and light control performance weakening of traditional photochromic probe in physiological environment Fig 2 UV / visible cyclic regulatory imaging (source: j.am Chem SOC.) co-author: Fu Youxin, Master graduate, Han haihao, Ph.D., Associate Professor Zhang Junji, he Xiaopeng, associate researcher, academician Tian He, East China University of science and technology, co-author of the paper, and guest professor Ben L Feringa Professor Li Na's guidance and support in SAXS technology were obtained This research work is supported by NSFC Science Center, key projects, NSFC excellent young scientists fund, 111 talent introduction program and other funds.