Journal of Chromatography A: purification of pomegranate tannin by two-dimensional molecularly imprinted solid-phase extraction

Molecularly imprinted is a new technology for the preparation of materials with specific selectivity according to given template, which is widely used in the enrichment and separation of various targets, including the separation and purification of effective and active components in natural drugs However, molecularly imprinted polymers have some technical limitations, which hinder their further application, mainly including three aspects: the selectivity of molecularly imprinted fillers is relatively simple, which often can not meet the separation needs of complex systems; the non-specific adsorption of non covalent molecularly imprinted polymers generally exists, which determines that they are more suitable for enrichment rather than purification; the synthesis of molecularly imprinted polymers is still in its infancy The application scale of molecularly imprinted technology is limited by the level of small batch experiments Rapid purification of polyphenols from pomegranate peel by semi preparative two-dimensional imprinting system combined with crystallization and reversed phase chromatography (source: Journal of Chromatography A) Recently, the researchers of Xinjiang Institute of physical and chemical technology, Chinese Academy of Sciences, Xinjiang Key Laboratory for the utilization of unique medicinal resources, started from the purification requirements of pomegranate tannins, respectively, by controlling the amount of initiators and a small number of batches, the scale-up synthesis of ellagic acid and anisidine imprinted polymers was realized; the imprinted polymers were filled in the semi preparation level respectively In the SPE column, the "two-dimensional" molecularly imprinted system was assembled In order to optimize the purification efficiency of "two-dimensional" molecularly imprinted system, the concept of "functional complementarity" suitable for molecularly imprinted evaluation was proposed based on the two-dimensional liquid chromatography orthogonality evaluation system, and the best purification condition of "ellagic acid - anisidine" two-dimensional molecularly imprinted system was finally determined Finally, the system was applied to the rapid separation of four tannin components (ellagic acid, anthocyanin, pomegranate A and ellagic acid hexoside) in the pomegranate peel extract Combined with the classical methods such as reversed-phase liquid chromatography and crystallization, the obtained components were secondary purified, and a better purity of pomegranate peel tannin was obtained The technology is fast, simple and has certain industrialization potential The concept of "functional complementarity" proposed in this paper can be used for reference in the condition optimization of two-dimensional molecular imprinting system Paper link: http: www.sciencedirect.com/science/article/pii/s0021967317307380