Liang Ruizheng, associate professor of Beihua and Yan Dongpeng, Professor of Beishi University: nano photosensitizers based on layered double hydroxides and isophthalic acid for singlet oxygen production and photodynamic therapy

At present, in photodynamic therapy, photosensitizers are limited by factors such as photoluminescence penetration, and their 1O2 production efficiency and therapeutic effect are limited The emergence of nanomaterials provides a new way to solve this problem Recently, Liang Ruizheng, associate professor of Beijing University of chemical technology and Yan Dongpeng, Professor of Beijing Normal University, reported a supramolecular photosensitizer excited by near-infrared light, which can enhance long-life three wire state excitation by using two-dimensional confinement effect and realize effective two-photon photodynamic therapy (NAT Commun 2018, 9, 2798) Gao Rui and Mei Xuan, Ph.D students of Beijing University of chemical technology, are working together for this paper Prof Liang Ruizheng, associate professor of Beijing University of chemical technology In June 2009, he obtained a bachelor's degree from Guangxi University, and in 2014, he was jointly trained as a doctor in Oxford University In June 2015, he obtained a doctor's degree from Beijing University of Chemical Technology (Duan Xue academician research group) In July 2015, he began to be an associate professor of the State Key Laboratory for the effective utilization of chemical resources of Beijing University of chemical technology The main research interest is the preparation of two-dimensional intercalation composite and its performance in biomedical field At present, as the first or corresponding author, he has published 25 research papers collected by SCI in the journals of NAT Commun., adv mater., adv funct Mater., biomaterials, chem SCI., chem Mater., etc., invited to write 3 reviews, including 5 if > 10, 14 If > 6, and cited more than 400 times As the person in charge, he has undertaken 5 projects including National Natural Science Foundation and Beijing Natural Science Foundation, and participated in 2 projects of "973 Plan" It has won the first batch of candidates of "100 young talents plan" of Beijing University of chemical technology Prof Yan Dongpeng, Professor of Beijing Normal University In 2007 and 2012, he obtained Bachelor of science and doctor of engineering degree from Beijing University of chemical technology, and studied with Academician Duan Xue In July 2012, he stayed in school and was employed as an associate professor In 2014, he was employed as a professor and doctoral supervisor of School of chemistry, Beijing Normal University The research fields are inorganic supramolecular chemistry, self-assembled ordered functional films, photoelectric function regulation of molecular crystalline materials, etc In the past five years, he has published more than 80 SCI papers (59 papers with if > 5, 18 papers selected as article covers or illustrations) in NAT Commin., angelw Chem Int ed., adv.funct Mater., chem SCI., mater Horiz., nano energy, small, Chem Commin And other journals as corresponding authors He cited more than 2800 times, and the h-index was 36 The research work was reviewed and reported by nature chemistry, nature China, ACS newsworthy chemistry, chemistry views, materials views and Science Daily in the form of "news and views" and "research highlight" As the co editor of the 166 volume "photofunctional layered materials" of the well-known academic series in inorganic and structural chemistry, the young Editorial Committee of China's Applied Chemistry and China Chemical express, the reviewer or arbitrator of more than 40 international academic journals, and the top 10 reviewer selected by cryengcomm in 2016 Two American invention patents and 18 national invention patents are authorized He has successively presided over three national natural funds and one Beijing natural fund, participated in major research projects of the Ministry of science and technology, and was selected as a new star of science and technology in Beijing He has won the honors of excellent doctoral thesis of Beijing awarded by Beijing Municipal Education Commission, "model of becoming a talent of Beijing University", excellent Communist Party member of Beijing University, etc Frontier research achievements: nano photosensitizers based on layered double hydroxides and isophthalic acid are used in the production of singlet oxygen and photodynamic therapy Singlet oxygen (1O2) has high activity and oxidation, and has great potential in the field of photodynamic therapy and photocatalytic oxidation Photosensitizer is an important reagent for the conversion of O2 to 1O2 In recent years, researchers have used graphene, silicon materials and black phosphorus as photosensitizers to produce 1O2, but the absorption range of these photosensitizers is usually below 700 nm, which will lead to poor penetration of stimulated light-emitting tissue, and then limit the production efficiency of 1O2 Therefore, it is very important and challenging to develop photosensitizers excited by near-infrared light for biological diagnosis and treatment Recently, Liang Ruizheng, academician Duan Xue of Beijing University of chemical technology, associate professor of Professor Wei Min's research group and Professor Yan Dongpeng of Beijing Normal University reported a kind of supramolecular photosensitizer excited by near-infrared light, which can realize effective two-photon photodynamic therapy by using two-dimensional limit enhanced long-life three line state excitation The singlet oxygen yield of the nanocomposite is as high as 0.74 The results of cell experiments in vitro showed that the anti-tumor effect of the nanocomposite was good, and the IC 50 was 0.153 μ g / ml In mouse experiments, the nanocomposites showed strong tissue penetration, low biological toxicity and strong tumor inhibition This work provides a new idea for the design of two-photon excited photosensitizers Fig 1 Nano composite as two-photon photosensitizer produces 1O2 (source: Nat Commun.) firstly, the author selects two-dimensional layered composite hydroxides (LDHs) as the main body and five organic small molecules as the object to prepare intercalated hydrotalcite nano composite materials by coprecipitation method Through the interlayer and spatial confinement effect of hydrotalcite, the organic phosphor unit is fixed in the rigid two-dimensional space, which greatly suppresses the energy loss caused by the rotation and vibration of the organic phosphor, thus improving the three-wire luminescence life Fig 2.1 the production and spectral characterization of O2 (source: Nat Commun.) under the excitation of two-photon light source with the same power, the hydrotalcite nanocomposites intercalated with isophthalic acid (IPA) showed the strongest luminous intensity, indicating that it had a very high quantum yield of triplex excitons At the same time, the author tested the fluorescence emission peak of 1O2 at 1270 nm by different characterization methods, The degradation of 3-diphenylisobenzofuran (dpbf) in UV absorption and ESR spectra show that m-phthalic acid intercalated hydrotalcite nanocomposites have the strongest 1O2 production ability and can be further used as photosensitizers Fig 3 Structure characterization of nanocomposites and the production efficiency of 1O2 (source: Nat Commun.) then, the author characterized the morphology of m-phthalic acid intercalated hydrotalcite nanocomposites, and found that they have two-dimensional lamellar structure, with a thickness of about 4 nm When the intercalation ratio of m-phenylenedicarboxylic acid reaches 100%, its 1 O 2 production efficiency is the highest Subsequently, the author measured the quantum yield of 1O2 of the nanocomposite with rose red as the standard reagent The results showed that the quantum yield of 1O2 of the photosensitizer is as high as 0.74 (rose red is 0.76), which is higher than most of the common photosensitizers at present Fig 4 Photodynamic therapy effect of IPA / LDH in HeLa cells (source: Nat Commin.) next, the author selected HeLa cells for cell experiment and found that the hydrotalcite nanocomposites intercalated with isophthalic acid showed good biocompatibility without light Under the light condition, the author used 2 ', 7' - dichlorofluorescein diacetate (DCFH-DA) indicator to detect the active oxygen in the cells The results showed that there was obvious 1O2 production in the cells MTT test showed that the IC 50 of the nanocomposite was 0.153 μ g ml − 1, which had excellent photodynamic effect More dead cells (red fluorescence) were observed in the results of calcein AM / PI staining, which further verified the excellent therapeutic effect of the light Figure 5 Results of biological distribution and pharmacokinetics in vivo (source: Nat Commun.) in order to further study the metabolism behavior of the photosensitizer in vivo, the author used cy5.5, a near-infrared dye, to label the photosensitizer, and studied its pharmacokinetics and distribution in vivo The results showed that the half-life of photosensitizer in mice was as high as 9.71 ± 0.93 h, which was beneficial to its circulation in vivo and accumulation of tumor site The results of near-infrared fluorescence imaging showed that: because of the existence of EPR effect, 12 hours after injection, the maximum accumulation of photosensitizers in the tumor site, and higher than other tissues and organs Figure 6 Photodynamic effect of IPA / LDH in vivo (source: Nat Commin.) finally, the author injected the photosensitizer into mice by tail vein injection, and observed the tumor growth of mice within 16 days The results of tumor photos and sections showed that m-phthalic acid intercalated hydrotalcite nanocomposites had a significant inhibitory effect on tumor, which proved its excellent photodynamic effect Nowadays, people and scientific research have been paid more and more attention in the economic life China has ushered in the "node of science and technology explosion" Behind the progress of science and technology is the work of countless scientists In the field of chemistry, in the context of the pursuit of innovation driven, international cooperation has been strengthened, the influence of Returned Scholars in the field of R & D has become increasingly prominent, and many excellent research groups have emerged in China For this reason, CBG information adopts the 1 + X reporting mechanism CBG information, chembeangoapp, chembeango official microblog, CBG wechat subscription number and other platforms jointly launch the column of "people and scientific research", approach the domestic representative research group, pay attention to their research, listen to their stories, record their demeanor, and explore their scientific research spirit.