Ma Huimin research group of Institute of chemistry developed an optical probe to detect the process of cell iron death

Iron death is a newly discovered regulatory cell death process, which is caused by iron dependent accumulation of lipid peroxides and is closely related to ROS As the most active ROS, hydroxyl radicals (· oh) have been proved to cause lipid peroxidation ·It can be assumed that · Oh is an important cause of lipid peroxidation in the process of iron death However, it is not clear how the level of · Oh changes during iron death In addition, the increase of cytoplasmic viscosity is one of the most typical morphological characteristics of apoptosis Due to the difference in morphology between iron death and apoptosis, it is of great significance to investigate whether iron death is accompanied by changes in cytoplasmic viscosity and its relationship with · oh and lipid peroxidation Based on the above problems, with the support of NSFC, the Ministry of science and technology and the Chinese Academy of Sciences, Shi Wen, Li Xiaohua and other researchers from the Ma Huimin research group of the Institute of chemistry of the Chinese Academy of sciences have developed the first dual-function fluorescent probe H-V that can simultaneously detect · oh and cytoplasmic viscosity The research results were published in J am Chem SOC (DOI: 10.1021 / Japs 9b09722) under the title of "ferrositis accredited by · Oh generation and cyclomatic visibility increase reviewed via dual functional fluorescence probe" On the one hand, the increase of viscosity can restrict the intramolecular rotation of H-V, resulting in the enhancement of fluorescence at 520 nm; on the other hand, the specific hydroxylation of H-V by · oh and subsequent structural rearrangement can lead to the expansion of conjugated system and the near-infrared fluorescence opening response at 652 nm Therefore, H-V can detect · oh and viscosity respectively in two non interfering signal channels The study of H-V fluorescence imaging showed that the process of cell iron death was accompanied by the formation of · Oh, the increase of cytoplasmic viscosity and the acceleration of lipid droplet formation Among them, the generated · Oh accounts for most of the total ROS, which may be the main cause of lipid peroxidation in the process of iron death, and ultimately lead to the increase of cytoplasm viscosity and the rapid formation of lipid droplets.