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The service life of a chromatographic column is not only related to the analyzed sample, mobile phase, and frequency of use, but most importantly, it is closely related to daily maintenance
1.
2.
3.
4.
After the buffer salt is precipitated, it is very difficult to remove it.
(1) Isocratic conditions
(2) Gradient conditions
(3) Pay attention to the following points in the process of using buffer
5.