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7.
1.
4.
2 Determination method
In the literature that has been published about NFs residue detection methods, most of the early reports are for the detection of original drugs
.
However, because NFs are sensitive to light and have the characteristics of rapid metabolism, the half-life in animals is only a few hours, and it is usually impossible to detect the original drug residues in animal tissues
(1) High performance liquid chromatography (HPLC)
There are few reports on the detection of NFs metabolites by liquid chromatography.
This is because conventional LC determination techniques cannot meet the sensitivity requirements for detecting NFs metabolites in edible tissues, but they can still be used for methodological research
.
Arancibia et al.
established a HPLC-UV detection method for nitrofurantoin and its metabolites in urine
.
This method uses SFE to extract nitrofurantoin and its metabolites in urine.
(2) Micellar electrokinetic capillary chromatography (MEKC)
Wickramanayake et al.
established a micellar electrokinetic capillary chromatography method for the determination of NFs and their metabolites (NFM) for the first time
.
Sodium deoxycholate (SDC) is selected as the surfactant to form micelles, and the central combined experimental design (CCD) method is adopted to optimize the separation conditions.
Related links: Maximum allowable residue limits of nitrofuran metabolites and analytical techniques-pretreatment methods (2)
