Nie Guangjun/Wang Hai, National Nanoscience Center, Chinese Academy of Sciences, "Nano Letters" Polyethyleneimine Graphene Oxide Hydration

Abstract: Messenger RNA (mRNA) vaccine can be used for cancer immunotherapy because it can encode tumor-associated antigens with excellent safety
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Unfortunately, RNA inherent instability and translation efficiency are the main limitations of RNA vaccines
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Recently, the Nie Guangjun/Wang Hai team of the National Nanoscience Center of the Chinese Academy of Sciences published a paper entitled In Situ Transforming RNA Nanovaccines from Polyethylenimine Functionalized Graphene Oxide Hydrogel for Durable Cancer Immunotherapy in Nano Letters
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Researchers report that at least 30 days after subcutaneous injection, an injectable hydrogel formed of graphene oxide (GO) and polyethyleneimine (PEI) can produce mRNA (ovalbumin, a model antigen) and adjuvant (R848) Contains nano vaccines
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The released nano-vaccine can protect mRNA from degradation and endow lymph nodes with targeted delivery capabilities
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The data shows that this transformable hydrogel can significantly increase the number of antigen-specific CD8 T cells, and then only one treatment is needed to inhibit tumor growth
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At the same time, this hydrogel can generate antigen-specific antibodies in the serum, thereby preventing the occurrence of transfer
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Overall, these results demonstrate the potential of PEI-functionalized GO transformable hydrogels in effective cancer immunotherapy
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? [Main image see analysis] Figure 1.
Schematic diagram and characteristics of the transformable hydrogel
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Figure 2.
In vitro translation and activation of immune cells for antigen presentation
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Figure 3.
Long-term release of antigen and adjuvant from GLP-RO gel
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Figure 4.
Tumor immunotherapy in vivo
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Figure 5.
In vivo metastasis prevention
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[Summary] The research team has developed a transformable hydrogel (GLP-RO gel) to release nanoparticles loaded with mRNA (mOVA) and adjuvant (R848) for long-term cancer immunotherapy
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This transformable hydrogel can not only encapsulate and protect mRNA from degradation, but also target lymph nodes to activate immune cells
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After subcutaneous injection, GLP-RO Gel can release GLP-RO NP for at least 30 days without obvious side effects
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Importantly, 30 days after in vivo injection, mOVA in the GLP-RO gel can still be detected
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As a result, GLP-RO gel can effectively inhibit tumor growth with only one treatment
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The mOVA and R848 in GLP hydrogel can effectively activate the immune system and produce tumor antigen antibodies in the serum, thereby effectively preventing the formation of metastases
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Overall, this study proves the great potential of GLP-RO gel in realizing long-lasting and effective cancer immunotherapy
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References: doi.
org/10.
1021/acs.
nanolett.
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