Non-steroidal anabolic hormone drug residue analysis pretreatment method-hydrolysis

9.
1.
4 Residue analysis technology

9.
1.
4.
1 Pre-processing method

The non-steroidal anabolic hormone drugs in biological samples often form conjugates with glucuronide and sulfuric acid.
These conjugates have high polarity and water solubility, poor thermal stability, and large differences in properties from the original drugs.
Loss, need to be hydrolyzed before processing
.

(1) Hydrolysis method

1) Enzymatic hydrolysis

The enzymatic hydrolysis of non-steroidal anabolic hormone drugs often uses arylsulfatase , glucuronidase and their mixtures
.

Yang et al.
used enzymatic hydrolysis to extract 50 anabolic hormone drugs (including DIS, HES, and DES) from muscle (pork, beef, and shrimp), milk, and liver
.

Peng Tao et al.
used enzymatic hydrolysis to extract RALs (TAL, a-ZOL, β-ZOL, ZER, ZAN, ZON) from animal liver
.

Kathrin et al.
used enzymatic hydrolysis to extract stilbene compounds (DES, DIS, HES) and RALs (ZER, TAL, a-ZOL, β-ZOL and ZON) in cow urine
.

2) Acid hydrolysis

There are also reports in the literature that non-steroidal anabolic hormone drugs are often combined with lipoproteins, so acid hydrolysis can improve the recovery rate
.