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The post-column derivatization technology uses derivatization reactions to cause a chemical reaction between the analyte and the corresponding reagents to change the chemical and physical properties of the analyte and enable it to be detected
1.
(1) Many selective reagents can be selected to make the analyte easy to detect
(2) Since the sample is separated first, the matrix of the sample is purified or retained on the chromatographic column before analysis, and only the purified sample is reacted, saving a lot of sample purification operations
Carbamate is a widely used insecticide.
The general structure of carbamate is N-methyl substituted carbamate with different ester-forming groups
2.
(1) Stability of reagents
(2) Reaction speed
(3) Reproducibility
(4) The lowest detection signal of the reagent
Figure 2-15 Reaction process of post-column derivatizer
(5) Solubility
(6) The inhomogeneity of the fluid, the baseline noise is related to the flow noise of the eluent and the reagent pump
(7) The excitation wavelength and reflection wavelength should be set correctly according to the detected compound
Application example: PICKERING post-column derivatization post-column conditions for carbamate analysis
Derivatization reagent 1: Hydrolysis derivatization reagent CB130
Derivatization reagent 2: 100mg o-phthalaldehyde (OPA) and 2g mercaptoethanol are dissolved in 950mL CB910
.
Reactor 1: 100°C, 0.
5 mL
.
Reactor 2: Ambient temperature
.
Derivatization reagent flow rate: 0.
3mL/min
.
Detection: Fluorescence detector, excitation wavelength λ ex =330nm, emission wavelength λ em =465nm
.
Carbamate analysis column 0846250 (4.
6mm×250mm), C 18 , 5um
.
The derivatization reagent used in carbamate analysis is the hydrolysis reagent NaOH
.
Note: During the first installation, reagent bottles, pipelines and pumps should be flushed with methanol to reduce possible fluorescent background
.
Related Links: Maintenance and Maintenance of Liquid Chromatography Columns
