Research group of Professor Gao Hui of Tianjin University of Technology: Biodegradable supramolecular materials for targeting Gram-positive bacteria and reducing antimicrobial resistance

The design of advanced biodegradable antibacterial materials and the development of new effective targeted antibacterial methods can not only reduce the pressure of multi drug resistance (MDR) bacterial infection, but also selectively inhibit a specific bacteria, which is expected to be further applied in clinical treatment In recent years, the research group of Professor Gao Hui of Tianjin University of science and technology has carried out a series of innovative research in the field of preparation of polymer antibacterial materials for drug-resistant bacteria Recently, Professor Gao Hui of Tianjin University of science and technology and Professor Yang Yingwei of Jilin university designed a biodegradable supramolecular material based on cationic polyaspartic amide derivative and column [5] arene (CP [5] a) through host guest interaction, and successfully applied it to target Gram-positive bacteria and reduce antimicrobial resistance Relevant research results were published in advanced functional materials (DOI: 10.1002/adfm.201904683) Professor Gao Hui's research group was founded in 2009 Focusing on the theme of biomedical self-assembly system and its functionalization, the research on targeted drug delivery and fluorescence imaging for tumors and pathogens was carried out in-depth, and a series of nanomaterials with good biocompatibility, high drug delivery ability and imaging effect were obtained In the past ten years, the research group has grown from its initial shape to today's growing strength, which is inseparable from the hard guidance and careful cultivation of the founder of the research group, Gao Hui During this period, many research results of the research group were published in the international famous magazines such as angelw.chem Int ed., adv funct Mater., chem Mater., ACS appl Mater Interfaces, chem Commun., polymer Chem., J mater Chem B The research team is mainly supported by the Ministry of education's "new century excellent talents support plan", six National Natural Science Fund projects, Tianjin "discipline leading talents training plan", Tianjin Natural Science Fund key projects and other funds At present, the research group has 2 doctoral students and 13 master students Our research group warmly welcomes the doctors, masters and undergraduates who are interested in biomedical polymer synthesis and performance research to join our family Introduction to Professor Gao Hui, Dr Gao Hui, distinguished professor of Tianjin, doctoral supervisor, deputy dean of School of chemistry and chemical engineering of Tianjin University of technology, in charge of scientific research of the college Graduated from Nankai University in 2005, and engaged in postdoctoral research at the University of Montreal in 2006-2009 The co tutor was Prof Jean Christophe Leroux Since taking part in the work, he has been committed to the design, synthesis and application of biomedical polymers Starting from the problems to be solved in clinical practice, a series of new functional polymers were designed and prepared, and the performance characteristics and application potential of the materials were systematically evaluated, and innovative research results were obtained The research work was successively supported by four national natural science projects and two Tianjin Natural Science Funds In 2011, he was selected into the JSPS Visiting Professor Award program, and conducted a 10-month visit in the research group of Professor Kataoka of Tokyo University In 2012, he was selected into the "new century excellent talents support program of the Ministry of education" and the "young and middle-aged backbone innovative talents training program" of Tianjin In 2015, he was selected as the first level candidate of "Tianjin 131 innovative talents training project", 2017 He was selected as "distinguished professor" in As an invited reviewer of journals such as acsapp Mater & Inter., mol Pharm., Biomacrolecules, j.control Release, EUR J Pharm Biopharm It pays attention to the cultivation of students' ability Many of the graduate students under guidance have won national scholarships and excellent master's thesis of Tianjin The undergraduates under guidance have won the second "Tianjin university student innovation and entrepreneurship scholarship"; the third prize of the 12th "Challenge Cup" national university student extracurricular academic and technological works competition, the special prize and the third prize of Tianjin university students extracurricular academic and technological works competition The 8th China Youth Science and Technology Innovation Award Cutting edge scientific research achievements: since the advent of penicillin in 1930s, the unscientific use and long-term overuse of antibiotics have led to the emergence of resistant bacteria, resulting in the decline or even failure of the efficacy of conventional antibiotics in many cases Therefore, it is urgent to develop more advanced antibacterial materials and explore various effective methods to inhibit pathogens, in order to reduce the widespread use of antibiotics, so as to reduce the pressure of multi drug resistance (MDR) bacterial infection In recent years, Professor Gao Hui's research group has made a series of progress in the field of preparation of polymer antibacterial materials for drug-resistant bacteria, including the preparation of mesoporous silicon polymer composite materials and the study of their antibacterial properties (ACS appl Mater Inter., 2015, 7, 17255-17263; polymer Chem., 2018, 9, 228-235), application of supramolecular nanocomposites in bacterial detection and bacteriostasis (ACS appl Mater Inter., 2017, 9, 10180-10189), biodegradable antibacterial polymer materials with bacterial detection function (chem Mater., 2018, 30, 1782-1790), etc In this paper, a kind of biodegradable supramolecular material based on cationic polyaspartic amide derivative and column [5] arene (CP [5] a) was designed, which was successfully applied to target Gram-positive bacteria and reduce antimicrobial resistance Through ring opening polymerization of β - benzyl-l-aspartic acid n-carboxylic anhydride (bla-nca), four kinds of degradable cationic polyaspartic amide derivatives (q-pasp (R)) with different side chains were synthesized by the research group of Professor Gao Hui and Professor Yang Yingwei of Jilin University By comparing their antibacterial properties, the author chose the polymer (q-pasp (BDA)) with side chain of 4-C chain for further study In order to improve the biocompatibility of q-pasp (BDA), a new antibacterial material (Q @ CP [5] a) targeting Gram-positive bacteria was obtained by introducing CP [5] a into macromolecular antimicrobial agents by supramolecular host guest method In addition, the author verified the effect of supramolecular complex Q @ CP [5] a through a series of experiments in vitro, and studied the wound treatment effect of gram-positive methicillin-resistant Staphylococcus aureus (MRSA, which is considered to be one of the most dangerous pathogenic bacteria in clinical treatment) infection in vivo, in order to further prove the practical application of Q @ CP [5] a (Figure 1) Fig 1 The construction diagram of the super molecular antibacterial system and its antibacterial performance for MRSA infected wound healing (source: adv funct Mater.) the rich cationic quaternary ammonium group in the polymer is helpful to insert the copolymer into the negative bacterial membrane, making the membrane break down, leading to the leakage of important substances in the bacteria and death In contrast to the Gram-negative bacteria with lipopolysaccharide linked cell outer membrane, the Gram-positive bacteria with negatively charged phosphobic acid are more likely to combine with the cationic polymer, so the antibacterial agent prepared by the author has a lower minimum inhibitory concentration (MIC) for gram-positive Staphylococcus aureus The anionic macroloop of CP [5] A and the thicker extracellular membrane of gram-negative E.coli prevent the biological complex Q @ CP [5] a loaded by CP [5] a from binding to the bacterial surface, so that Q @ CP [5] a can selectively inhibit Staphylococcus aureus (Fig 2) It should be noted that Q @ CP [5] a not only has a strong membrane damaging effect on MRSA, but also has the biodegradability of polyaspartic amide to avoid the occurrence of antimicrobial resistance Fig 2 (a) plate chart of S aureus and E coli; (b) statistical chart of S aureus colony; (c) statistical chart of E coli colony; (d) scanning electron micrograph of S aureus and E coli (source: adv funct Mater.) The author also compared the resistance of vancomycin and antibacterial materials to MRSA through drug resistance test: on the 16th day, the MIC of vancomycin to MRSA reached 2048 times of that of the first day, while the MIC of antibacterial materials to MRSA remained the initial mic, because the degradable skeleton of polymer avoided the generation of bacterial resistance (Figure 3) Figure 3 Mic test of vancomycin, q-pasp (BDA) and Q @ CP [5] a added to MRSA for continuous culture for 16 days (source: adv funct Mater.) in general, the author designed a cationic polyaspartic amide derivative and column [5] Aromatic hydrocarbon is a biodegradable supramolecular material, which can not only target Gram-positive bacteria, but also reduce antimicrobial resistance This simple and novel antibacterial system has a biodegradable framework, strong selective antibacterial activity and the ability to promote wound healing, which is expected to be further applied in clinical treatment The research results were recently published in advanced functional materials (DOI: 10.1002/adfm.201904683) The first author of this paper is Yan Shuzhen, a 2017 level postgraduate of School of chemistry and chemical engineering of Tianjin University of technology, and Chen Shuai, a 2016 level postgraduate The corresponding authors are Professor Gao Hui, Professor Chen Li of Tianjin University of technology and Professor Yang Yingwei of Jilin University The research was funded by general program of National Natural Science Foundation of China (21674080), Tianjin distinguished professor, Tianjin leading talent plan, key program of Tianjin Natural Science Foundation (No 18jczdjc37700) and Tianjin University Innovation Team Training Program (td13-5020) Nowadays, people and scientific research have been paid more and more attention in the economic life China has ushered in the "node of science and technology explosion" Behind the progress of science and technology is the work of countless scientists In the field of chemistry, in the context of the pursuit of innovation driven, international cooperation has been strengthened, the influence of Returned Scholars in the field of R & D has become increasingly prominent, and many excellent research groups have emerged in China For this reason, CBG information adopts the 1 + X reporting mechanism CBG information website, chembeangoapp, chembeango official micro blog, CBG information wechat subscription number and other platforms jointly launch the column of "people and scientific research", approach the representative research groups in China, pay attention to their research, listen to their stories, record their demeanor, and explore their scientific research spirit.