Research group of Professor Jiang Zhengjin of Jinan University: application of new biomimetic polymer materials to biological separation of C-reactive protein in biological fluid

C-reactive protein (CRP) is a non-specific inflammatory biomarker, which is the most powerful predictor and risk factor of cardiovascular disease However, due to the contamination of recombinant CRP or commercial CRP by Nan 3 and endotoxin, the physiological function of CRP in health and disease is controversial In order to study the mechanism of the interaction between CRP and disease, analyze the signal pathway more accurately, and obtain high-purity CRP with complete structure and function is very important At present, the purification materials of CRP tend to have strong nonspecific adsorption, and the additional purification steps in the purification process are likely to lead to the decrease of sample recovery or the introduction of new impurities Recently, Jiang Zhengjin, Professor of Jinan University, inspired by the specific combination of CRP and cell membrane under the condition of calcium ion to activate the complement system, developed a new bionic polymer material for the efficient biological separation of CRP in biological fluid The biomimetic material has the advantages of high recovery rate and good specific selectivity for CRP enrichment, which is obviously superior to the commercial CRP enrichment material It has been successfully used in the high-efficiency capture of CRP in the serum of inflammatory patients and mice The results were published in ACS appl Mater Interfaces (DOI: 10.1021 / acsami 8b15581) Introduction to Professor Jiang Zhengjin's research group there are 1 Professor, 4 high-end foreign experts, 5 associate professors, 2 postdoctors and 30 graduate students in the research group The research group is mainly engaged in the preparation of new materials, multi-dimensional and micro scale separation analysis, biomedical sensor and other modern analysis technologies, as well as the application research in the material basis of natural product efficacy, biopharmaceutical, drug target screening or mechanism of action Prof Jiang Zhengjin, Professor, doctoral supervisor, talent winner of the Ministry of education in the new century, leader of the pharmaceutical analysis discipline of Jinan University, assistant to the dean of the school of pharmacy, director of the pharmaceutical analysis research center At present, more than 90 papers have been published in the mainstream SCI journals such as angelw Chem Int ed., chem SCI., anal Chem., anal Chim Acta., J Chromatogr A He was awarded "excellent teacher of Guangdong Province" and "excellent graduate tutor of Jinan University" Currently, he is the editorial board member or young editorial board member of five journals such as J Pharm Biomed Anal., the authoritative journal of pharmaceutical analysis SCI, the chairman of the 27th Annual Conference of international pharmaceutical analysis and biopharmaceutical analysis, the standing member of the international PBA Committee (the only Chinese representative among the 15 members), the standing member of the pharmaceutical analysis technology branch of China Pharmaceutical Biotechnology Association, and has long served as the PBA, ISC Members of the International Scientific Committee of the mainstream academic conferences in pharmaceutical analysis industry such as APA, international invited review experts of NCN in Poland and fwo foundation in Belgium, and joint training tutors for doctoral students from the 11th University of Paris, the University of Liege in Belgium and the University of Alcala in Spain, etc Cutting edge research achievements: the application of new biomimetic polymer materials in the biological separation of C-reactive protein in biological fluid is inspired by the interaction between CRP and cell membrane in the presence of calcium ions The author selected 2 - (methacryloxy) ethyl phosphorylcholine (MPC), which has strong anti cell adhesion ability, as the monomer, with high hydrophilic n, Using n '- dimethylbisacrylamide (MBA) as crosslinking agent, a new kind of biomimetic polymer material for selective capture of CRP in biological fluid was developed by in-situ polymerization in the tip of the pipette gun using free radical polymerization (Fig 1) The biomimetic polymer material was characterized by SEM, EDS and FT-IR to confirm that the polymer was successfully prepared and the internal polymerization was uniform The results of pore size distribution show that there are 3 μ m pores in the polymer, which is conducive to the rapid protein transport in the polymer (Fig 2) The results of hydrophilic angle test show that the polymer has very high hydrophilicity, which can effectively inhibit the non-specific adsorption of protein on the surface of the material, so as to further improve the specificity and selective capture of CRP Fig 1 Development of biomimetic polymer material and its application in biological separation (source: ACS appl Mater Interfaces) Fig 2 Characterization of biomimetic polymer material (source: ACS appl Mater Interfaces) after systematic optimization of sample loading, elution and elution conditions, the author will combine the polymer material with commercial immobilized P -A systematic comparative study was carried out on the purified material of aminophenylphosporyl choline gel (Fig 3) The results showed that the purification effect of biomimetic polymer material for CRP in spiked serum was better than that of commercial purified material and the dynamic adsorption capacity was as high as 53.5 mg / g regardless of whether the adsorbent was incubated with the test sample or not At the same time, the biomimetic polymer still showed good protein recovery and purification effect after being used more than 100 times in 180 days In addition, the integrity of the structure and function of the purified CRP was confirmed by circular dichroism, fluorescence and immunobiochemical analysis Figure 3 Systematic comparative study of biomimetic polymer materials and commercial CRP purification materials (source: ACS appl Mater Interfaces) Finally, the author used biomimetic polymer materials for the enrichment and purification of CRP in the actual biological fluid samples (Figure 4) The first choice was the serum samples of patients with inflammation (the total protein concentration was 91 mg / ml), in which the CRP concentration was 0.05mg/ml, it was found that the recovery rate of biomimetic polymer was 100% and the concentration purity was 95% In order to further verify the selection ability of biomimetic polymer, the author selected mouse serum samples with total serum protein concentration of 186 mg / ml (CRP concentration of 0.06 mg / ml) as the research object, and also found that biomimetic polymer can effectively capture mouse CRP with a purity of 95% The above results were further confirmed by MALDI-TOF MS Figure 4 Practical application of biomimetic polymer materials in biological fluid samples (source: ACS appl Mater Interfaces) This achievement was recently published on ACS appl Mater Interfaces (DOI: 10.1021 / acsami 8b15581) The first communication unit of this paper is Jinan University The first author is Dr Wang Qiqin and master student Jin Hanying The corresponding author is Dr Wang Qiqin Professor Jiang Zhengjin of Jinan University and Professor Gan Ning of Ningbo University The above research work has been successively supported by general program of NSFC, special program of China Postdoctoral Science Foundation and general program Nowadays, people and scientific research have been paid more and more attention in the economic life China has ushered in the "node of science and technology explosion" Behind the progress of science and technology is the work of countless scientists In the field of chemistry, in the context of the pursuit of innovation driven, international cooperation has been strengthened, the influence of Returned Scholars in the field of R & D has become increasingly prominent, and many excellent research groups have emerged in China For this reason, CBG information adopts the 1 + X reporting mechanism CBG information, chembeangoapp, chembeango official microblog, CBG wechat subscription number and other platforms jointly launch the column of "people and scientific research", approach the domestic representative research group, pay attention to their research, listen to their stories, record their demeanor, and explore their scientific research spirit.