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Sodium taurocholate
Sodium Taurocholate
【Structural formula】
[Molecular formula and molecular weight] C 26 H 14 NO 4 NaS; 537.
【Source】Dry gallstones of Bos Taurus domesticas Gmelin
[Properties] Off-white crystalline powder
This product is soluble in methanol
Melting point: 232~234℃
【Purity Check】
TLC
1.
Developing solvent n-butanol - acetic acid - water (10:1:1)
Check the color development of 10% sulfuric acid ethanol solution, heat at 105℃, observe under sunlight
2.
Developing solvent of isopropanol - acetate - methanol - water (10:2:3:1)
Check the color development of 10% sulfuric acid ethanol solution, heat at 105℃, observe under sunlight
High performance liquid chromatography
Chromatographic column Phenomenex C 18 , 5pm (4.
Mobile phase acetonitrile-0.
Detection wavelength 207nm
【Structural identification】202
3416, 2945, 2870, 1660, 1550, 1460, 1380, 1200, 1050, 1085
FAB-MS m/z: 538[M] + , 516,392,308,290
1 H-NMR (CD 3 OD) δ: 0.
13 C-NMR (CD 3 OD) δ: 31.
【Storage】Dry and airtight