Tetracycline residue analysis technology-determination of thin layer chromatography

(3) Thin layer chromatography (TLC)

TLC is a chromatographic analysis method with simple equipment, convenient operation, fast separation speed, high sensitivity and resolution
.

(4) Flow-injection analysis (FIA)

FIA is a quantitative flow analysis technology that can get rid of the balance theory of solution chemical analysis, and can reproduce the sample and reagent zone in the liquid flow under non-thermal equilibrium conditions.
It has high precision, fast analysis speed and easy implementation.
Features such as automation
.

Wang Hao et al.
used FIA-chemiluminescence (CL) method to determine DC in blood and urine samples
.

(5) Capillary electrophoresis (capillary electrophoresis, CE)

CE has the advantages of simple operation, no sample contamination of the chromatographic column, fast analysis speed, high separation efficiency, low sample consumption, and low operating cost
.

Chen Tianbao and others used CE to separate and determine TC, OTC, DC and CTC in honey
.

Kowalski used CE with a bubble detection cell to detect TC, OTC and DC in fish samples within 6 minutes
.

The method uses a quartz capillary column (57cm×75umi.
d.
), injection pressure of 3.
45kPa for 5s, separation voltage of 20kV, separation temperature of 22℃, UVD detection at 270～280nm
.

The separation buffer contains 30mmol/L sodium phosphate, 2mmol/L Na ₂ EDTA and 2.
5% 2-propanol (pH12), separation voltage is 14kV, dynamic injection is 5s, 360nm measurement
.

Nozal et al.
reported a method for the determination of TC, OTC and DC in water samples using CE
.

Mu et al.
established a CE analysis method for TC, OTC and DC residues in milk
.