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    Home > The following three key points should be grasped about the use of full exhaust biosafety cabinet

    The following three key points should be grasped about the use of full exhaust biosafety cabinet

    • Last Update: 2018-02-07
    • Source: Internet
    • Author: User
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    As a widely used laboratory equipment, full exhaust biosafety cabinet should master the following three key points: first, the location of the biosafety cabinet is about 0.45m/s from the opening in the front of the biosafety cabinet into the cabinet At this wind speed, the consistency of one-way flow in the cabinet is easy to be disturbed, and it is easy to be disturbed by the walking, opening windows, air supply and distribution fans and door switches of people near the safety cabinet According to the ideal arrangement, it should be placed away from activities and places that may interfere with air flow Try to leave a space of 30cm at the back and both sides of the safety cabinet for maintenance Above the cabinet, a height of 30-35cm is left to measure the wind speed of the exhaust filter and leave enough space for the replacement of the exhaust filter II Decontamination treatment of biosafety cabinet decontamination of level 1 and level 2 biosafety cabinets is to place an appropriate amount of oligoformaldehyde (the concentration of oligoformaldehyde in the air inside the cabinet is 80%) on the electric heating plate or frying pan (controlled from outside the cabinet) In addition, an electric hot plate or frying pan containing 10% more hydrogen carbonate than the oligoformaldehyde is also placed in the cabinet (controlled outside the cabinet) The second container should have a cover that can be removed from a distance (e.g tied to a wire that can be pulled from outside the cabinet) In this way, the oligoformaldehyde neutralized in advance can reach Zui small If the relative temperature is lower than 70%, open container with hot water If there is no front partition, the raw plastic baffle shall be used, and it shall be taped on the front face to ensure that no gas will leak into the room When the oligoformaldehyde in the disk has evaporated completely or the switch has been turned on for 1 hour, turn off the switch Keep the cabinet untouched all night Then remove the cover of the second plate and turn it on and off, and the ammonia bicarbonate begins to evaporate At this time, turn off the switch of the plate, let the safety cabinet start to work, and make the hydrogen carbonate ammonia cycle for 1 hour The front partition (or plastic baffle) can then be removed and the safety cabinet can be used III cleaning and disinfection of biosafety cabinet all articles in the biosafety cabinet, including equipment, shall be decontaminated on the surface and taken out from the cabinet after the completion of the work, because the remaining culture medium may provide the growth conditions of microorganisms The internal surface of the safety cabinet shall be decontaminated before and after each use To wipe the surface and inner wall of the worktable with disinfectant, the disinfectant used shall be able to kill all microorganisms that may exist in the cabinet At the end of the day, the surface shall be decontaminated, that is, the workbench, all sides, back and inside of the glass shall be fully wiped 70% alcohol can be used, which is an effective disinfectant for the target organism If this kind of corrosive disinfectant is used, it should be wiped twice with sterilized water It is recommended that the working cabinet be in working condition during cleaning and disinfection If it is not in working condition, it shall be operated for 5 minutes to remove the gas in the safety cabinet before it is closed Shanghai Sujing Industry Co., Ltd if you have any need, you can pass with us at any time Thank you very much for your support and look forward to cooperating with you Thank you & bestragard! Suing Jinghua
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