Tips for using sepabean ® machine fast liquid chromatograph in summer

The hot summer of Applied Technology Research Center is like fire, and the iron plate baking in the laboratory ~ for many synthetic laboratories, summer is the most difficult season Air conditioning suck no force, reaction needs to control temperature and other factors, causing a steamer in the laboratory It's said that Xiaobian was also tempered in such an environment, so I would like to pay high tribute to the researchers who are still fighting in the front line in the hot summer season! So, when it comes to the matters needing attention when using sepabean ® machine rapid liquid chromatograph for sample separation and purification in high temperature environment, today's editor is here to share a little experience for you, hoping to bring you a comfortable coolness like cold drink I don't know if you find this situation when the indoor temperature is high: when using petroleum ether or dichloromethane as the mobile phase, there are often many bubbles in the system pipeline, resulting in a lot of burrs in the spectrum during the separation process, or even the system can't pump liquid In fact, this situation is generally low boiling point organic solvents "the back of the pot." Let's take a look at the physical properties of petroleum ether: petroleum ether is a colorless liquid with kerosene odor, mainly a mixture of pentane and hexane, generally with boiling range specifications of 30 ℃ ~ 60 ℃, 60 ℃ ~ 90 ℃, 90 ℃ ~ 120 ℃ Special attention should be paid to the parameter of "boiling range" Because petroleum ether itself is a mixture, petroleum ether with different components has different boiling range If you choose petroleum ether with boiling range of 30 ℃ ~ 60 ℃ as eluent in high temperature summer, you should pay more attention to solvent bottle and separation column If there are a lot of bubbles, some measures should be taken The feasible options are: 1) turn on the air conditioner to reduce the ambient temperature; 2) choose petroleum ether with higher boiling range, or simply change other organic solvents with higher boiling point as the mobile phase In addition to the above two methods, Santai technology also provides users with the third option: optional online degassing module With this "artifact", there is no need to worry about the problem of bubbles entering the system pipeline! At present, the R & D engineers of Santai technology are working overtime I believe that in the near future, the majority of users can see it in the list of options Having said petroleum ether, let's talk about dichloromethane, which is also a common through column solvent used in organic synthesis laboratory The boiling point of dichloromethane at atmospheric pressure is 39.8 ℃, which, like the petroleum ether with low boiling range, also causes the problem of air bubbles in the system pipeline at high temperature In addition, the adsorption process of dichloromethane and silica gel packing in the normal phase column is an exothermic process, which often causes a large number of bubbles in the separation column under high temperature environment In order to avoid this problem, we suggest that you should select a lower flow rate to slowly moisten the column in the moistening stage before sample separation, so that the heat generated in the separation column can be effectively released In order to ensure that the subsequent separation experiments can be carried out smoothly Remember one thing: you can't eat hot tofu in a hurry! Well, here are the tips for the use of sepabean ® machine fast liquid chromatograph in this issue I hope you can keep a cool heart and successfully complete all the experimental tasks in the hot summer More exciting content, please look forward to the next update!