UV detector light source life is limited, how can I better control the cost of purification preparation? ——Time collection mode for the introduction of new functions of sepabean ® machine

As one of the core modules of the rapid liquid chromatography system, the UV detector module of Xubo applied technology research center is the foundation of on-line monitoring in the process of sample separation The light source of UV detector usually includes mercury lamp, deuterium lamp, xenon lamp, light-emitting diode, etc sepabean ® machine produced by Santai technology uses deuterium lamp as the UV light source, which can realize the continuous adjustable and full band scanning detection function of 200 ~ 400 nm band However, as a general consumable, the service life of deuterium lamp is about 2000 ~ 5000 hours In the continuous sample separation process of a large number of samples, the UV detector module is normally open to monitor the elution process in real time In this case, the loss of deuterium lamp is a continuous process, which increases the cost of preparation Let's imagine such a scenario: researchers need to purify a large number of samples, and need to repeatedly inject and separate samples on the flash separation column of conventional size At this time, the separation and purification method has reached a high repeatability after optimization Under this condition, if component collection can be conducted according to the fixed time period, then real-time monitoring without UV detector can be realized Control can also achieve the purpose, so as to effectively control the preparation cost In response to the above practical problems, the researchers of Santai technology developed a new function for sepabean ® machine: time collection mode, and added the function of closing the UV detector in the instrument control software In order to verify the practical effect of the new function, Xiaoqian, an application engineer, used the synthetic products of organic small molecules as samples to carry out the verification experiment The sample is the product of the last step of a synthesis reaction, and the reaction formula is shown in Figure 1 Figure 1 Synthesis reaction formula of the sample The sample has strong polarity and is suitable for reverse phase separation First, HPLC analysis is carried out with C18 reverse phase analysis column, and the analysis spectrum is shown in Figure 2 According to the results of HPLC, the retention time of the target product was 4.249 min Fig 2 Next, Xiaoqian started flash preparation 8.0 g of the sample to be purified was dissolved in 40 ml of methanol aqueous solution (V: v = 3:1) The sample was completely dissolved by ultrasonic heating The maximum size of C18 reversed-phase flash separation column of normal size in the laboratory is 330 g considering the sample quantity, the sample quantity is 10 ml each time during flash preparation The experimental parameters of flash preparation are shown in Table 1 Table 1 Sepabean ® machine chromatographic column 330g sepaflash ® bonded series C18 reversed-phase purification column (spherical silica gel, 20-45 μ m, 100 μ m, order No.: sw-5222-330-sp) detection wavelength 220 nm; 254 nm mobile phase solvent A: water; solvent B: methanol flow rate 40 ml / min injection volume 10 ml elution gradient time (min) Solvent B (%) Figure 3 shows the flash preparation map of two consecutive injections It can be seen from the analysis map that the retention time and peak shape of the target product are highly consistent Therefore, in the subsequent two injections, the component collection mode is changed to the time collection mode, and the fixed collection time is set from 22 min to 29 min at the same time, the deuterium lamp of the UV detector is turned off Under this condition, the collected components were analyzed by HPLC, and the analysis map is shown in Figure 4 Figure 4 HPLC analysis of purified sample According to figure 4, the target product obtained in time collection mode also has a high purity (99.5%) Therefore, under the premise of highly repeatable separation method, without the on-line monitoring of UV detector, the same preparation and purification effect can be obtained through target product collection in a preset time period as in the usual threshold collection or peak collection mode This case shows that the cost of preparation can be further effectively controlled by turning off the UV detector combined with time collection mode without losing the effect of sample preparation and purification For the majority of researchers, it is undoubtedly of great economic significance Santai technology always takes the interests of customers as the highest standard, adheres to the concept of "creating extra value for customers", and will continue to launch various new products and solutions in the future, please look forward to! To learn more about the detailed specifications of sepabean ® machine, or the flash column ordering information used with it, please visit CBG online store: https://store.chembergo.cn/.